8,720 results

1. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCGN-ATF6

   Plasmid

   #11974

   Purpose

   For mammalian expression of human ATF6 with an HA tag

   Depositor

   Ron Prywes

   Article

   Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. MCS-13X Linker-BioID2-HA

   Plasmid

   #80899

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL13 promoter luciferase

   Plasmid

   #11784

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-13 promoter (IL13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRY2PHR-mCherryN1

   Plasmid

   #26866

   Purpose

   Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2PHR (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS2-123

    Plasmid

    #26598

    Depositor

    Michael Elowitz

    Article

    Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

    Insert

    Synthetic
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-pLKO-sgRNA-puro

    Plasmid

    #104321

    Purpose

    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

    Depositor

    Nathanael Gray

    Article

    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Beta-Catenin-20

    Plasmid

    #55001

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Beta-Catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyCatcher003

    Plasmid

    #133447

    Purpose

    Expresses SpyCatcher003 in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbi-GP

    Plasmid

    #60511

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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