Addgene

We narrowed to 22,251 results for: Sele

Showing: 1951 - 1980 of 22251 results

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pGTag NGFR EGFP nLAP -0

Plasmid

#194505

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -2

Plasmid

#194513

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 V192S

Plasmid

#175769

Purpose

Lentiviral vector expressing N-terminally tagged GFP-TAX1BP1 V192S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

V192S

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -1

Plasmid

#194512

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP cLAP -0

Plasmid

#194511

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP +1

Plasmid

#194509

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP nLAP -1

Plasmid

#194506

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP cLAP +0

Plasmid

#194508

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP +2

Plasmid

#194310

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP +0

Plasmid

#194308

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP -2

Plasmid

#194313

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP -0

Plasmid

#194311

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP +1

Plasmid

#194309

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP nLAP +1

Plasmid

#194315

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP nLAP +0

Plasmid

#194314

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP cLAP -1

Plasmid

#194312

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2_SulR(CDS)

Plasmid

#186423

Purpose

provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Cds (part b3-b4-b5), goldenbra…

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP129

Plasmid

#189537

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-129-GFP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-3×FLAG-SLC35A2

Plasmid

#186283

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1023-Tier1-PhCMV-p2A-PuroR

Plasmid

#169497

Purpose

Tier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-PuroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1021-Tier1-PhCMV-PuroR

Plasmid

#169492

Purpose

Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven puromycin N-acetyl-transferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5b_Ptet

Plasmid

#179338

Purpose

gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a_Ptet

Plasmid

#179337

Purpose

gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHAGE-eGFP-TAX1BP1 Y635A

Plasmid

#175772

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y635A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-eGFP-TAX1BP1 N637A

Plasmid

#175773

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

N637A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pStr-KDEL_APV-SBP-Dendra2-ER-5

Plasmid

#170679

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

APV-SBP-Dendra2-ER-5
streptavidin-KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

IRES and T7

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCm N-term SMT3 NikJC199U

Plasmid

#174210

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Use

Tags

8xHis, SMT3, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCm C-term SMT3 NikJC199U

Plasmid

#174362

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Use

Tags

8xHis, SMT3, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1951 - 1980 of 22251 results

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