We narrowed to 22,160 results for: ESC

1. px459-AMBRA1 #3

   Plasmid

   #172606

   Purpose

   Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTol2-UAS-zmiRFP

   Plasmid

   #108415

   Purpose

   Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zmiRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-EF1a-Archon2-KGC-EGFP-ER2

   Plasmid

   #108426

   Purpose

   Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon2-KGC-EGFP-ER2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmiRFP-beta-Actin-C-19

   Plasmid

   #108412

   Purpose

   Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   beta-Actin
   

   Use

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-2xFLAG-2xSTREP-CDK4

   Plasmid

   #172615

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSF143-wArchon2-KGC-EGFP-ER2

   Plasmid

   #108430

   Purpose

   Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   wArchon2-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   flp-18

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-2xFLAG-2xSTREP-CCND3

   Plasmid

   #172623

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-2xFLAG-2xSTREP-CCND2

   Plasmid

   #172629

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179429

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-CCND1(T286A)

    Plasmid

    #172631

    Purpose

    Expresses untagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CaMKII-Archon1-KGC-EGFP

    Plasmid

    #108418

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA528 IST1 L328D (316-366)(C-Cys)

    Plasmid

    #193035

    Purpose

    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, Residues 316-366, Non-native C-terminal Cy…

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 IST1 L355A (316-366)(C-Cys)

    Plasmid

    #193036

    Purpose

    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L355A, Residues 316-366, Non-native C-terminal Cy…

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-SET-EMD (N-SET-EMD)

    Plasmid

    #217765

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226)

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSET-EMD-EGFP (C-SET-EMD)

    Plasmid

    #217764

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226)

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217755

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pKSR1-CA3-GS-mRFP1 (C-KSR-GS)

    Plasmid

    #217758

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 with GGSSGGGGA linker

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKSR1-CA3-GS-EGFP (C-KSR-GS)

    Plasmid

    #217757

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 with GGSSGGGGA linker

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

    Plasmid

    #207493

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIG-851_HA-GD2-28z_HA-TFAP4

    Plasmid

    #207501

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px458-AMBRA1 #3

    Plasmid

    #172607

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA5-LRRK2

    Plasmid

    #229019

    Purpose

    Expression of untagged full length human LRRK2 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    no tags (untagged)

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDisplay-iSeroSnFR

    Plasmid

    #128484

    Purpose

    Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Use

    Tags

    Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV(MinDis).iGluSnFR

    Plasmid

    #41732

    Purpose

    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    Myc, PDGFR transmembrane helix (aa 513-561), and …

    Expression

    Mammalian

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    CMV

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIG-702_CD19-BBz_CAR_tNGFR

    Plasmid

    #207485

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-BBz_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIG-704_CD19-28z_CAR_tNGFR

    Plasmid

    #207486

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

    Plasmid

    #207487

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

    Plasmid

    #172640

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-GRAB_DA1m

    Plasmid

    #113049

    Purpose

    express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Has Service

    AAV9

    Insert

    GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLPC-JAG1-mVenus

    Plasmid

    #171170

    Purpose

    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIG-874_HA-GD2-28z_CAR_IL2RA

    Plasmid

    #207504

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    IL2RA, HA-GD2-28z_CAR (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pIG-671_HA-GD2-28z_CAR_tNGFR

    Plasmid

    #207484

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTol2-UAS-zArchon1-KGC-EGFP-ER2

    Plasmid

    #108427

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon1-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-hSyn-GRAB_DA1h

    Plasmid

    #113050

    Purpose

    express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

    Plasmid

    #172611

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDKN1B (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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41. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pAAV-CAG-Archon1-KGC-EGFP-ER2

    Plasmid

    #108420

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-CAG-iSeroSnFR-Nlgn

    Plasmid

    #128485

    Purpose

    Fluorescent reporter for serotonin (dendrite localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Has Service

    AAV9

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Full-length neuroligin, Ig-kappa leader, and cpsf…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

    Plasmid

    #207491

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

    Plasmid

    #108422

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV5

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217769

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400

    Promoter

    EF-1alpha promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid

    #155189

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    beta-catenin (Ctnnb1 Mouse)
    

    Use

    Unspecified; Piggybac transposon

    Tags

    P2A eGFP NLS

    Expression

    Mammalian

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pmiRFP-alpha-Tubulin-19-C

    Plasmid

    #108413

    Purpose

    Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    alpha-tubulin b (Tuba1b Mouse)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pKSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217753

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge