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We narrowed to 5,531 results for: PEP

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Showing: 3251 - 3300 of 5531 results

  1. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid
    #214207
    Purpose
    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lyn A (LYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214207-plasmid-map-sequence-id-422791

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  2. pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

    Plasmid
    #214231
    Purpose
    Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Grb2 SH2 domain (GRB2 Human)
    Tags
    6xHis, AviTag, and mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214231-plasmid-map-sequence-id-424761

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  3. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid
    #214203
    Purpose
    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lck kinase SH2 domain (LCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214203-plasmid-map-sequence-id-422773

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  4. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid
    #214204
    Purpose
    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Blk kinase SH2 domain (BLK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214204-plasmid-map-sequence-id-422774

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  5. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid
    #214206
    Purpose
    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    p60-HCK (HCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214206-plasmid-map-sequence-id-422790

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  6. pcDNA3-P-SSP-AAA Mutant

    Plasmid
    #238432
    Purpose
    Express amino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)
    Depositor
    Amy Shiu Lee
    Article
    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:
    Insert
    amino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP) (HSPA5 Human)
    Expression
    Mammalian
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238432-plasmid-map-sequence-id-482340

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  7. pcDNA3-P-SSP-Wild-Type

    Plasmid
    #238431
    Purpose
    Express amino acid 631-650 of Wild-type GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)
    Depositor
    Amy Shiu Lee
    Article
    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:
    Insert
    amino acid 631-650 of Wild-type GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP) (HSPA5 Human)
    Expression
    Mammalian
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238431-plasmid-map-sequence-id-482338

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  15. A1-LCD allW D262V

    Plasmid
    #234616
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234616-plasmid-map-sequence-id-469043

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  16. A1-LCD allW D262N

    Plasmid
    #234615
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234615-plasmid-map-sequence-id-469028

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  17. *MtbCM-HiBiT-His

    Plasmid
    #228028
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available Since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    228028-plasmid-map-sequence-id-451757

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  18. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  19. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Tags
    6xHis Tag
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226455-plasmid-map-sequence-id-458394

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  20. pAAV hGRN4

    Plasmid
    #213684
    Purpose
    Expresses human granulin-4 with an N-terminal twin-Strep-FLAG tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human granulin-4 (hGRN4) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and FLAG tag (after signal peptide…
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213684-plasmid-map-sequence-id-455752

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  23. pDR111_mannose_Stat1Klf6

    Plasmid
    #188400
    Purpose
    Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Stat-1 (Stat1 Mouse, Escherichia coli)
    Klf6 (Klf6 Mouse)
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188400-plasmid-map-sequence-id-370468

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  26. pcDNA3-sACE2v3

    Plasmid
    #145150
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 3
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145150-plasmid-map-sequence-id-292586

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  28. pcDNA3-sACE2v4

    Plasmid
    #145151
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 4
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145151-plasmid-map-sequence-id-292588

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  29. pcDNA3-sACE2v6

    Plasmid
    #145153
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 6
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145153-plasmid-map-sequence-id-292591

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  34. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  36. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  40. pcDNA3-sACE2v2.4(732)-IgG1

    Plasmid
    #154106
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available Since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154106-plasmid-map-sequence-id-301643

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  42. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87379-plasmid-map-sequence-id-212256

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  44. pcDNA3-sACE2v2.4(732)-8h

    Plasmid
    #154103
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-G…
    Promoter
    CMV
    Available Since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154103-plasmid-map-sequence-id-301646

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  49. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available Since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

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  50. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available Since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

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Showing: 3251 - 3300 of 5531 results