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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,524 results for: piggyBac

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Showing: 1301 - 1320 of 1524 results

  2. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

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  3. XLone-BSD Cas13d P2A mCherry

    Plasmid
    #155183
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Unspecified; Piggybac transposon
    Tags
    NLS and P2A mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155183-plasmid-map-sequence-id-307948

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  10. GWF180

    Plasmid
    #133607
    Purpose
    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9
    Depositor
    Dustin Maly
    Publication
    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    Expression
    Mammalian
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    133607-plasmid-map-sequence-id-261658

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  15. Vector 1174D

    Plasmid
    #200012
    Purpose
    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)
    Depositor
    Omar Akbari
    Publication
    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.
    Insert
    Engineered dobulesex splicing module
    Use
    Synthetic Biology
    Expression
    Insect
    Mutation
    Engineered dobulesex splicing module
    Promoter
    Hr5IE1
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200012-plasmid-map-sequence-id-395199

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  16. pSCL.139

    Plasmid
    #184994
    Purpose
    Express Cas9-P2A-Eco1RT in human cells
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-P2A-Eco1RT
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Human codon optimized RT
    Promoter
    TetOn-3g
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184994-plasmid-map-sequence-id-376613

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  17. XLone-Cas9-2A-GFP

    Plasmid
    #170159
    Purpose
    Express Cas9 and GFP upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Publication
    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    Synthetic Biology; Piggybac
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    TRE3G promoter
    Available since
    July 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    170159-plasmid-map-sequence-id-334930

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  19. pb-CAGGS-dCas9-VP64-blasto

    Plasmid
    #218556
    Purpose
    Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Publication
    Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS-VP64
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218556-plasmid-map-sequence-id-431934

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Showing: 1301 - 1320 of 1524 results