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Showing: 3301 - 3350 of 9181 results

  3. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  6. pRYG

    Plasmid
    #113642
    Purpose
    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113642-plasmid-map-sequence-id-214560

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  14. pCRISP_IS

    Plasmid
    #120425
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120425-plasmid-map-sequence-id-240993

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  15. p005kanRFP

    Plasmid
    #58533
    Purpose
    RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58533-plasmid-map-sequence-id-90572

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  16. pTU2-a-RFP

    Plasmid
    #72958
    Purpose
    Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - a site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  21. pTU1-B-RFP

    Plasmid
    #72940
    Purpose
    Level 1 Destination vector - Position B
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - B site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72940-plasmid-map-sequence-id-447507

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  25. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  30. pY1

    Plasmid
    #50597
    Purpose
    Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Type
    Empty backbone
    Use
    Synthetic Biology; Empty vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLacUV5
    Available since
    Oct. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pKB237

    Plasmid
    #170030
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 (CCL19 Human, Escherichia coli, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    170030-plasmid-map-sequence-id-335295

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  38. pTU1-A-lacZ

    Plasmid
    #72935
    Purpose
    Level 1 Destination vector - Position A
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - A site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72935-plasmid-map-sequence-id-447506

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Showing: 3301 - 3350 of 9181 results