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DinoIII-bsr

Plasmid

#155027

Purpose

Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Blasticidin-S deaminase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Promoter

FCP

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBBR1MCS-2

Plasmid

#85168

Purpose

Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

Depositor

Kenneth Peterson

Article

Kovach et al Gene. 1995 Dec 1;166(1):175-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Expression

Bacterial

Promoter

Constitutive (derepressed P-lac)

Available Since

Nov. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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PB-TetR-tdTomato

Plasmid

#212958

Purpose

piggyBac donor vector containing TetR-tdTomato for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetR-tdTomato

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-ER-5

Plasmid

#55294

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ER (CALR Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-cilia-APEX

Plasmid

#73186

Purpose

Expresses cilia-APEX in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

cilia-APEX (Nphp3 Glycine max, Mouse)

Tags

internal GFP tag

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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Full Length Rat Bassoon

Plasmid

#250160

Purpose

Expresses Rat Bassoon in mammalian cells

Depositor

Thomas Dresbach

Article

Kapoor et al Elife. 2026 Jan 13;13:RP98254. doi: 10.7

Insert

Bassoon (Bsn Rat)

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

Plasmid

#60629

Purpose

Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 human MyoD (MYOD1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CD36-C-10

Plasmid

#55280

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD36 (CD36 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

Plasmid

#182344

Purpose

sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Spacer 4

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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