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Plasmid#215887PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-99a-I-type
Plasmid#215888PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_4
Plasmid#210630PurposeLuciferase experiment for TAL1 promoter 4DepositorInsertpromoter 4 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
FENEF-Sensor
Plasmid#208930Purposein vitro p38-Sensor for luminescent detection of p38-activityDepositorInsertFENEF sequence from human ATF2 with phospho site (ATF2 Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_5
Plasmid#210632PurposeLuciferase experiment for TAL1 promoter 5DepositorInsertpromoter 5 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_exon 4
Plasmid#210631PurposeLuciferase experiment for TAL1 exon 4DepositorInsertTAL1 exon 4 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-B4GALT3mut
Plasmid#203602PurposeReporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.DepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLuciferaseTagsExpressionMammalianMutationSeed sequences in miR-27b target sequences were m…PromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1mut
Plasmid#203604PurposeReporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequencesDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseTagsExpressionMammalianMutationmiR-27b target sequences in the 3'UTR was mu…PromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.15_pT-REX17
Plasmid#195497PurposeFirefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17DepositorInsertPromoter region of lncRNA T-REX17
UseTagsluc2-hPESTExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-WT
Plasmid#190094Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR Wild Type (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-1
Plasmid#190095Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-1 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 6704-6709 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-2
Plasmid#190096Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-2 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 7122-7129 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA Td2
Plasmid#176258PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA Td1
Plasmid#176257PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA Td2
Plasmid#176255PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR2
Plasmid#176245PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR3
Plasmid#176246PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsa-cdk2 3'UTR sicheck2 mut
Plasmid#121124Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
UseTagsExpressionBacterialMutationmiR-199 seed binding site mutatedPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2 199 mut
Plasmid#121122Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
UseTagsExpressionBacterialMutationmiR-199 seed binding site mutatedPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2
Plasmid#121123Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR (cdk2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hARPC2
Plasmid#112549Purpose3'UTR reporter assayDepositorInsertARPC2 (ARPC2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 - mut 3'UTR
Plasmid#97161PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationmiR-722 Binding MutantPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-CALB2-3'UTR mir-30 mut1
Plasmid#74428PurposeCalb2 UTR with mutated mir-30 site 1DepositorInsertCALB2 3'UTR (CALB2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-CALB2-3'UTR delta ARE
Plasmid#74425PurposeCalb2 UTR with mutated AU binding sequenceDepositorInsertCALB2 3'UTR (CALB2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc
Plasmid#73038PurposeExpress Nluc reporterDepositorInsertNluc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1S-Nluc
Plasmid#73033PurposeExpress NlucDepositorInsertNluc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterTruncated EF1Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE4 (plus strand)
Plasmid#91850PurposeLuciferase reporter for IL2RA enhancer (IGI-P0345)DepositorInsertIL2RA CaRE4 (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE4 scramble (plus strand)
Plasmid#91852PurposeLuciferase reporter for IL2RA enhancer (IGI-P0347)DepositorInsertIL2RA CaRE4 scramble (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationSequence scrambled from ch10:6094588-6094934PromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE4 (minus strand)
Plasmid#91849PurposeLuciferase reporter for IL2RA enhancer (IGI-P0626)DepositorInsertIL2RA CaRE4 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE1 (plus strand)
Plasmid#91837PurposeLuciferase reporter for IL2RA enhancer (IGI-P0614)DepositorInsertIL2RA CaRE1 (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE3 (plus strand)
Plasmid#91844PurposeLuciferase reporter for CD69 enhancer (IGI-P0621)DepositorInsertCD69 CaRE3 (plus strand) (CD69 Human)
UseLuciferaseTagsExpressionMammalianMutationMissing 1 base from 13 base polyT tract beginning…PromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE5 (plus strand)
Plasmid#91841PurposeLuciferase reporter for IL2RA enhancer (IGI-P0618)DepositorInsertIL2RA CaRE5 (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE3 (minus strand)
Plasmid#91835PurposeLuciferase reporter for IL2RA enhancer (IGI-P0612)DepositorInsertIL2RA CaRE3 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs7893324 A>G, rs7090530 G>T, rs7090512 A&g…PromoterAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE3 (minus strand)
Plasmid#91845PurposeLuciferase reporter for CD69 enhancer (IGI-P0622)DepositorInsertCD69 CaRE3 (minus strand) (CD69 Human)
UseLuciferaseTagsExpressionMammalianMutationMissing 1 base from 13 base polyT tract beginning…PromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE4 SNP (plus strand)
Plasmid#91851PurposeLuciferase reporter for IL2RA enhancer (IGI-P0346)DepositorInsertIL2RA CaRE4 SNP (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs61839660 C>TPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE5 (minus strand)
Plasmid#91836PurposeLuciferase reporter for IL2RA enhancer (IGI-P0613)DepositorInsertIL2RA CaRE5 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE2 (plus strand)
Plasmid#91846PurposeLuciferase reporter for IL2RA enhancer (IGI-P0623)DepositorInsertIL2RA CaRE2 (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs6602425 T>G, T>C at chr10:6117603PromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE2 (minus strand)
Plasmid#91847PurposeLuciferase reporter for IL2RA enhancer (IGI-P0624)DepositorInsertIL2RA CaRE2 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs6602425 A>C, G>A mutation at chr10:611706…PromoterAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE6 (minus strand)
Plasmid#91840PurposeLuciferase reporter for IL2RA enhancer (IGI-P0617)DepositorInsertIL2RA CaRE6 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs7893467 C>APromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE6 (plus strand)
Plasmid#91839PurposeLuciferase reporter for IL2RA enhancer (IGI-P0616)DepositorInsertIL2RA CaRE6 (plus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationrs7893467 G>T, rs11256448 A>GPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-IL2RA CaRE1 (minus strand)
Plasmid#91838PurposeLuciferase reporter for IL2RA enhancer (IGI-P0615)DepositorInsertIL2RA CaRE1 (minus strand) (IL2RA Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shLuc
Plasmid#110422PurposeshLuc control, silence Luc gene, doxycycline inducible, puromycin selectionDepositorInsertLuciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pB-TopFlash-NanoLucP
Plasmid#204347PurposeTopFlash reporterDepositorInsertTopFlash-NanoLucP
UseLuciferaseTagsNanoLucPExpressionMutationPromoterminiPAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR100
Plasmid#180377PurposepAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).DepositorInsertsNFAT-Luciferase
hCD8A
UseTagsExpressionBacterial and MammalianMutationPromoterhEF1-HTLV and minimal promoter and an NFAT respon…Available sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHCAG-L2EOP
Plasmid#51783PurposeThe oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expressionDepositorInsertsLuciferase-2A-EBNA-1
OriP
UseHerpes simplex virus type-1 amplicon vectorTagsExpressionMutationPromoterCAGAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-eGFP
Plasmid#104834PurposeeFFly luciferase for invivo Imaging, eGFP for flourescent detectionDepositorInsertseFFly
eGFP
UseLentiviral and Luciferase; EgfpTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only