Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 117,330 results for: CIL

Showing: 1361 - 1380 of 117330 results

  3. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

    Enlarge

  5. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

    Enlarge

  7. CoV2-N-P.1

    Plasmid
    #177959
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P80R,R203K,G204R
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177959-plasmid-map-sequence-id-348182

    Enlarge

  8. 4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172228
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells
    Depositor
    Joshua Rosenthal
    Article
    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis and Flag
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172228-plasmid-map-sequence-id-339581

    Enlarge

  9. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Tags
    Expression
    Mutation
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

    Enlarge

  16. CoV2-N-R203K

    Plasmid
    #177951
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (R203K) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R203K
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177951-plasmid-map-sequence-id-348191

    Enlarge

  18. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Use
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

    Enlarge

  19. pK1E-RNAP

    Plasmid
    #48144
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase K1E
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48144-plasmid-map-sequence-id-69927

    Enlarge

Showing: 1361 - 1380 of 117330 results