We narrowed to 2,923 results for: COB;

1. HIR WT

   Plasmid

   #24049

   Depositor

   Frederick Stanley

   Article

   Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

   Insert

   Insulin Receptor (INSR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SaCas9

   Plasmid

   #188494

   Purpose

   Expresses FLAG tagged SaCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AsCas12a

   Plasmid

   #188492

   Purpose

   Expresses FLAG tagged AsCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Plasmid L5 attB::Pleft* mWasabi

   Plasmid

   #169409

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mWasabi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Plasmid L5 attB::Pleft* mScarlet

   Plasmid

   #169410

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mScarlet proper
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSUM-Kan-MCS2-gfp

   Plasmid

   #109290

   Purpose

   Expresses high levels of gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Kanamycin

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HIR K1030R

    Plasmid

    #24050

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K1030R

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gh22

    Plasmid

    #106695

    Purpose

    expression of gRNA targeting COSMC

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    COSMC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pNHEJ-recAmu-sacB

    Plasmid

    #158717

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R61C

    Promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Plasmid L5 attB:Psmyc double green

    Plasmid

    #169412

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mWasabi
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Plasmid L5 attB:Psmyc triple red

    Plasmid

    #169413

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet proper
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. miniCMV eGFP hPGK BSD

    Plasmid

    #188519

    Purpose

    Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTW2065

    Plasmid

    #236574

    Purpose

    ISYmu1 PDS3 gRNA2 TRV2 Architecture_A

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBa.Kif1a 1-396.GFP

    Plasmid

    #45058

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

    Insert

    Kif 1a (Kif1a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    motor domain aa 1-396

    Promoter

    Chicken Beta Actin

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

29. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. gh19

    Plasmid

    #106703

    Purpose

    expression of gRNA targeting GALNT19

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GALNT19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HBT_pcoCASphi_version2

    Plasmid

    #197932

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    2xNLS-2xFLAG

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HIR Y1158,1162,1163F

    Plasmid

    #24054

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y1158F, Y1162F, Y1163F

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

35. dCas12j2 KRAB

    Plasmid

    #188508

    Purpose

    Expresses FLAG tagged dCas12j2 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV5-myc-Erk2

    Plasmid

    #39222

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2001 Jul 13;276(28):26509-1

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

37. LbCas12a

    Plasmid

    #188493

    Purpose

    Expresses FLAG tagged LbCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

39. gh40

    Plasmid

    #106714

    Purpose

    expression of gRNA targeting ST6GAL1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST6GAL1 (ST6GAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HIR Y1158F

    Plasmid

    #24051

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y1158F

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

41. HIR Y1162F

    Plasmid

    #24052

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y1162F

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

42. HIR Y1163F

    Plasmid

    #24053

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y1163F

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

43. NpT7-5-ERK1

    Plasmid

    #39229

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

44. pEGFP.N3-SLC35A2-GFP

    Plasmid

    #186284

    Purpose

    transient expression of C-terminal GFP tagged SLC35A2 isoform c/UGT1 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

    Insert

    SLC35A2 (SLC35A2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

47. dCas12j3 KRAB

    Plasmid

    #188507

    Purpose

    Expresses FLAG tagged dCas12j3 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSUM-Kan-MCS1-gfp

    Plasmid

    #109288

    Purpose

    Expresses high levels of gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    Multiple Cloning Site and GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBbdCas9S_P0526-sgRNA500

    Plasmid

    #149641

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBbdCas9S(RBSmut)

    Plasmid

    #149574

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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