We narrowed to 6,273 results for: nls

Showing: 2761 - 2800 of 6273 results

Results per page:

Sort results by:

pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-dCas9-PBE

Plasmid

#98159

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p4459 FLAG-hPLIC-1 NTF

Plasmid

#8664

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal fragment: amino acids 1-112 of hPLIC-1…

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OmEF1aCas9P2ApuroSB

Plasmid

#165484

Purpose

Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Insert

Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)

Use

CRISPR; Transposon

Promoter

Oreochromis mossambicus EF1 alpha promoter (OmEF1…

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
A3Bmax

Plasmid

#207167

Purpose

Express full-length human A3B in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Tags

NLS and NLS, 6x His

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2283

Plasmid

#70702

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Expression

Plant

Promoter

Ubi

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
A3A-PBE-ΔUGI

Plasmid

#119770

Purpose

Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-ΔUGI-NLS

Tags

NLS

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM2-rPMCA2a

Plasmid

#47762

Purpose

mammalian expression of rat PMCA2a

Depositor

John Penniston, Emanuel Strehler

Article

Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

Insert

PMCA2a (Atp2b2 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pET42b-A3A-PBE-ΔUGI

Plasmid

#119771

Purpose

To express the recombinant APOBEC3A in vitro

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS

Tags

6*HIS and NLS

Expression

Bacterial

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2266

Plasmid

#70705

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89872

Purpose

expresses CB1R-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

CB1R-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD38-52

Plasmid

#98685

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 38-52, residues 1853-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Tags

NLS-GFP

Expression

Yeast

Promoter

Pnmt1

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1277

Plasmid

#111101

Purpose

Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51D gRNA (RAD51D Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-37

Plasmid

#98686

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 27-37, residues 1773-1852

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_nucLAMA-G97 IRES mYFP

Plasmid

#130713

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS IRES mYFP

Tags

NLS and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_nucLAMA-F98 IRES mYFP

Plasmid

#130712

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS IRES mYFP

Tags

NLS and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGT003_nuclear-mKate2

Plasmid

#248085

Purpose

Constitutive expression of NLS-tagged mKate2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mKate2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGT002_nuclear-EGFP

Plasmid

#248084

Purpose

Constitutive expression of NLS-tagged EGFP from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

EGFP

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGT001_nuclear-mOrange2

Plasmid

#248083

Purpose

Constitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mOrange2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFC482

Plasmid

#246439

Purpose

The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

CasE

Tags

NLS

Expression

Mammalian

Mutation

SapI domesticated

Promoter

EF-1a

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP110_AAV Scramble Control

Plasmid

#239419

Purpose

Negative control AAV vector for SaCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble control

Use

AAV

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN103

Plasmid

#233860

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN104

Plasmid

#233861

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL007

Plasmid

#233866

Purpose

MoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2761 - 2800 of 6273 results

Results per page:

Sort results by:

More Filters

We narrowed to 6,273 results for: nls