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Addgene

We narrowed to 8,814 results for: CAG

Showing: 3451 - 3500 of 8814 results
  1. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212830
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.
    Depositor
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212831
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.
    Depositor
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pJ175

    Plasmid
    #29493
    Depositor
    Insert
    annexin V-128 (ANXA5 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Promoter
    Available since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only
  5. p426_Cas9_gRNA-ARS511b

    Plasmid
    #87387
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS511b sequence CAGTGTATGCCAGTCAGCCA in yeast chromosome 5.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS511b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV Syn Barcode22

    Plasmid
    #226193
    Purpose
    Expression mapping
    Depositor
    Insert
    Syn Barcode22
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. SGEP-sh-Hs-PHGDH-1956

    Plasmid
    #188670
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pENTRY4_ WRKY28_2

    Plasmid
    #126900
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pmCherry_I3

    Plasmid
    #239340
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mCherry in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pI3_EGFP

    Plasmid
    #239335
    Purpose
    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pmBaoJin_I3

    Plasmid
    #239337
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mBaoJin in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mBaoJin
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pmStayGold_I3

    Plasmid
    #239336
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pmScarlet3_I3

    Plasmid
    #239342
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mScarlet3
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pmScarlet-I_I3

    Plasmid
    #239341
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pmRuby3_I3

    Plasmid
    #239344
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. pmScarlet3-H_I3

    Plasmid
    #239343
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mScarlet3-H (a.k.a. mYongHong)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. pmStayGold_mStayGold_I3

    Plasmid
    #239339
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. pmStayGold(E138D)_I3

    Plasmid
    #239338
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Insert
    I3-01
    Use
    Tags
    mStayGold(E138D)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. OgeuIscB GCA RNA

    Plasmid
    #222864
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCA target
    Depositor
    Insert
    OgeuIscB omega RNA with a (GCA)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  21. OgeuIscB AGC RNA

    Plasmid
    #222866
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a AGC target
    Depositor
    Insert
    OgeuIscB omega RNA with a (AGC)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  22. pgTS40

    Plasmid
    #169630
    Purpose
    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.
    Depositor
    Insert
    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 / PCAG
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pKPI-MtLAP1

    Plasmid
    #193521
    Purpose
    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots
    Depositor
    Inserts
    MtLAP1
    DsRed
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    ATUBI10 and Medtr8g059790(KPI)
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3451 - 3500 of 8814 results