164,577 results

1. pBCW

   Plasmid

   #62658

   Purpose

   Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL-FLKif3A

   Plasmid

   #13742

   Depositor

   Linda Wordeman

   Article

   Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

   Insert

   mouse Kif3A (Kif3a Mouse)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT4/HB

   Plasmid

   #108352

   Purpose

   Optimized Sleeping Beauty Transposon Vector with MCS

   Depositor

   Wolfgang Uckert

   Article

   Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE LOX Cloning template vector

   Plasmid

   #139651

   Purpose

   Cloning template for ORANGE-CAKE conditional knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ePE6d

   Plasmid

   #229029

   Purpose

   Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

   Depositor

   Li Juan

   Article

   Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-PD-L1-SNAP

   Plasmid

   #223577

   Purpose

   Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ERG_pet28a

   Plasmid

   #131642

   Purpose

   Bacterial expression of ETS gene ERG

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ERG (ERG Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) K-Ras(G12C)

   Plasmid

   #224284

   Purpose

   Cell transfection and expression of K-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   K-Ras (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-zTRAP

   Plasmid

   #42236

   Depositor

   Stephen L Johnson

   Article

   Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

   Insert

   EGFP-Rpl10a (rpl10a Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mStayGold-EFS-Zeo

    Plasmid

    #227258

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK7-HA-S6K1-E389-deltaCT

    Plasmid

    #8993

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-SA-2A-NEO-CAG-RTTA3

    Plasmid

    #60431

    Purpose

    neomycin resistance; constitutive promoter driving reverse tet activator

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    rtTA3
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-hsRPA14 (MSW#503)

    Plasmid

    #208069

    Purpose

    Expression of GFP-tagged hsRPA3 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA3 (RPA3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-Talin

    Plasmid

    #80026

    Purpose

    Mammalian expression of mCherry-Talin

    Depositor

    Chinten James Lim

    Article

    Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

    Insert

    Talin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-NES-ZapCV2-Zinc dead

    Plasmid

    #203850

    Purpose

    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWS175

    Plasmid

    #90962

    Purpose

    Cas9 gap repair vector - HygR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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