Addgene

We narrowed to 84,526 results for: MAL

Showing: 2841 - 2880 of 84526 results

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EGFP-miSFIT-2x

Plasmid

#124691

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDG279

Plasmid

#85219

Purpose

mammalian expression of ubiquitin-K48R-EGFP fusion

Depositor

Douglas Gray

Article

Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

Insert

ubiquitin C (UBC Human)

Use

Tags

6xHIS and EGFP

Expression

Mammalian

Mutation

K48R

Promoter

human ubiquitin C

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pLEX-TCRb-FKBP-Blasticidin

Plasmid

#120723

Purpose

Expresses ER-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

T cell receptor β chain

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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miR190 in pcDNA3.1

Plasmid

#78122

Purpose

Mammalian expression of miR190

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR190 (MIR190A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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p068 pCS mTRPS1 (M)

Plasmid

#11040

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Two Cys residues at positions 917 and 920 in the …

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-P4:gs:nls:FKBP

Plasmid

#135984

Purpose

Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the FKBP domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTW063

Plasmid

#115950

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 SGP (+/- Apt) Dest

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8.3

Plasmid

#121959

Purpose

Mammalian expression, Transcription regulation, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, MS2 hairpin inserted

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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GWF303

Plasmid

#133611

Purpose

CD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CD95-1-2xPP7, PCP-DNCR2, BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GWF314

Plasmid

#133610

Purpose

CXCR4-com, NLS-com-GNCR1-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CXCR4-6-com, com-GNCR1, BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Raf1RBD

Plasmid

#120707

Purpose

Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Raf1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Raf1 amino acids 1-149

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTW322

Plasmid

#115953

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 With 3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW323

Plasmid

#115954

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 No 3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW325

Plasmid

#115956

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 With 3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW326

Plasmid

#115957

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 No 3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW327

Plasmid

#115958

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 End

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW328

Plasmid

#115959

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P3 With E1-3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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MSRP4-bio-His

Plasmid

#50806

Purpose

Expresses enzymatically monobiotinylated full length MSRP4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSRP4

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4 rat NZAP

Plasmid

#45908

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

Zc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMCh-1468

Plasmid

#82418

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M4

Plasmid

#80937

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-PTPRJi1

Plasmid

#59608

Purpose

Expression of shRNA against human PTPRJ

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRJ (PTPRJ Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM21i2

Plasmid

#59605

Purpose

Expression of shRNA against human ADAM21

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM21 (ADAM21 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-SARS2-Spike-HiBiT

Plasmid

#191212

Purpose

Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

Depositor

Mohan Babu

Insert

SARS-CoV-2 spike_WT (S SARS CoV-2)

Use

Tags

HiBiT (high affinity small nanoluciferase fragmen…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUB_SMFLuc0---MS2

Plasmid

#210568

Purpose

Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_SMFLuc100---MS2

Plasmid

#210570

Purpose

Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.PEX 1-42-tdTM-FKBP

Plasmid

#224415

Purpose

Mammalian expression of Pex3

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Pex3 (Pex3 Mouse)

Use

Tags

tdTom-FKBP

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-SauriCas9-KKH-BlastR

Plasmid

#220972

Purpose

Expresses human codon-optimized SauriCas9-KKH

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9-KKH

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-Sa-SlugCas9-BlastR

Plasmid

#220968

Purpose

Expresses human codon-optimized Sa-SlugCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Sa-SlugCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP-tHe-Tub5

Plasmid

#201738

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) epsilon-tubulin Tub5 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub5

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ptHe-Tub3-mScarlet

Plasmid

#201735

Purpose

Tardigrade (Hypsibius exemplaris) gamma-tubulin Tub3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin gamma

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ptHe-Tub2D4-mScarlet

Plasmid

#201734

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D4 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D4

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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