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Plasmid#162999Purposeplasmid expressing eABEDepositorInserteABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9992-HF-ABE
Plasmid#163000Purposeplasmid expressing HF-ABEDepositorInsertHF-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9995-evoABE
Plasmid#163003Purposeplasmid expressing evoABEDepositorInsertevoABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9784-xABE
Plasmid#163005Purposeplasmid expressing xABEDepositorInsertxABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvCYP76AD1
Plasmid#162529PurposeBeta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertCYP76AD1
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MjcDOPA5GT
Plasmid#162530PurposeMirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertcDOPA5GT
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN2-exon7-ESE1
Plasmid#126042PurposeIn-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN2 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF152_Phsp_Cas9
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRTagsExpressionWormMutationPromoterPhsp-16.48Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5WY
Plasmid#165050PurposeIntegrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)
Plasmid#164047PurposeCRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA geneDepositorInsertmCherry-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26
Plasmid#149346PurposeMammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNADepositorInsertCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbcAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-BbsI
Plasmid#149347PurposeMammalian expression vector for SpyCas9 and puromycin resistanceDepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbhAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE477
Plasmid#153268PurposeM3 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE478
Plasmid#153269PurposeM4 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE501
Plasmid#153274PurposeM7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE499
Plasmid#153272PurposeM6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE498
Plasmid#153271PurposeM5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL714
Plasmid#140384PurposeExpresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoterDepositorInsertT7-ctcO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL720
Plasmid#140390PurposeExpresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoterDepositorInsertT7-qacO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL722
Plasmid#140392PurposeExpresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoterDepositorInsertT7-ttgO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW065
Plasmid#115952PurposeDestination VectorDepositorInsertLvl0 3'UTR Dest
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH50
Plasmid#128212Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK007
Plasmid#128220Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH72
Plasmid#128221Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH73
Plasmid#128222Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH74
Plasmid#128223Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW103
Plasmid#115946PurposeORF insert for replicon MoCloDepositorInsertLvl0 L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW086M
Plasmid#115934PurposeORF insert for replicon MoCloDepositorInsertLvl0 mKate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m2
Plasmid#84721PurposepVR2 but with a G to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at position 16 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m3
Plasmid#84722PurposepVR2 but with a C to G mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationC to G mutation at position 22 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW087
Plasmid#115935PurposeORF insert for replicon MoCloDepositorInsertLvl0 EBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW085
Plasmid#115933PurposeORF insert for replicon MoCloDepositorInsertLvl0 mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOXA3-donor
Plasmid#112321PurposeCRISPR donor plasmid to tag human transcription factor FOXA3 with GFPDepositorInsertFOXA3 homology arms flanking EGFP-IRES-Neo cassette (FOXA3 Human)
UseCRISPRTagsExpressionMutationhg38:19:45872401:G>T, hg38:19:45872421:C>T,…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
AX44_pS.Donor.S1
Plasmid#100289PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas45
Plasmid#82390PurposesgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas39
Plasmid#82389PurposesgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas36
Plasmid#82388PurposesgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas35
Plasmid#82387PurposesgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas28
Plasmid#82385PurposesgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23108Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas27
Plasmid#82384PurposesgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23117Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas15
Plasmid#82383PurposesgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas14
Plasmid#82382PurposesgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only