We narrowed to 124,065 results for: CIL

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pMRX-IB-mRuby3-FTH1

Plasmid

#192804

Purpose

Stable expression of mRuby3-FTH1 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

FTH1 (FTH1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Mouse, Glycine max)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-HyPer-DAAO-NES

Plasmid

#119164

Purpose

Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

HyPer-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P199L

Plasmid

#177949

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P199L) (N )

Expression

Mammalian

Mutation

P199L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMA3287

Plasmid

#46883

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase, Y147A mutant (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Y147A

Promoter

TRE-Tight

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-SAKS1-Strep-HA

Plasmid

#113487

Purpose

Expression of human SAKS1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

SAKS1 (UBXN1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHT-6xR.ads-PxylA-DI

Plasmid

#47386

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

ads
dxs
idi

Tags

6xR and His

Expression

Bacterial

Promoter

Pgrac and PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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TALED_Right-Cox3.1-1397C-AD.

Plasmid

#212953

Purpose

ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-Cox3.1-1397C-AD

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Left-Cox3.1-1397N.

Plasmid

#212950

Purpose

Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-Cox3.1-1397N

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD4-Strep-HA

Plasmid

#113480

Purpose

Expression of human UBXD4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD4 (UBXN2A Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTF023_His_hsDDA1

Plasmid

#174480

Purpose

Expresses DDA1 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DDA1 (DDA1 Human)

Tags

His-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMA3288

Plasmid

#46885

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase WT (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

TRE-Tight

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-EGFP-SOS1

Plasmid

#178860

Purpose

Mammalian expression of SOS1 fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

SOS1 (SOS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His-MBP-NLS-RfxCas13d-NLS-HA

Plasmid

#171586

Purpose

Bacterial expression of NLS-RfxCas13d-NLS-HA

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

His-MBP-NLS-RfxCas13d-NLS-HA

Tags

HA

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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