We narrowed to 28,416 results for: REP

1. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

   Plasmid

   #164447

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

   Insert

   GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
   

   Use

   Tags

   GB1-His6-TEV and Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine, Cysteine 813 to Serine, S…

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

   Plasmid

   #62576

   Purpose

   Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   RASA1 (RASA1 Human)
   

   Use

   Luciferase

   Tags

   None

   Expression

   Mutation

   miR-206 binding site mutated (ACATTCCA --> AAC…

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

   Plasmid

   #128605

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   24xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

   Plasmid

   #169076

   Purpose

   Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Use

   Tags

   GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

   Plasmid

   #141371

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   C145A, human codon optimized

   Promoter

   EF1alpha

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166013

   Purpose

   mammalian expression of MERS-CoV soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   MERS-CoV S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166012

   Purpose

   mammalian expression of SARS-CoV soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

   Plasmid

   #31481

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce target) EF BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry contains M9S and M16L to reduce backgroun…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166015

    Purpose

    mammalian expression of HCoV-OC43 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-OC43 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166014

    Purpose

    mammalian expression of HCoV-HKU1 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-HKU1 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166017

    Purpose

    mammalian expression of HCoV-NL63 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-NL63 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166016

    Purpose

    mammalian expression of HCoV-229E soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-229E S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

    Plasmid

    #31483

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Traffic Light 2.0 series have a +3 PEST sequence …

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167971

    Purpose

    Tp63 reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    Tp63 (TP63 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167943

    Purpose

    SFTPC reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SFTPC (SFTPC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    EF1a

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

    Plasmid

    #68714

    Depositor

    Yutaka Hata

    Insert

    H2B mCherry and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

    Plasmid

    #128609

    Purpose

    Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    uORF1-36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

    Plasmid

    #31480

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (ani target)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S, M16L to reduce background mCherr…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge