We narrowed to 5,599 results for: SUP

1. Cx43K258stop-msfGFP

   Plasmid

   #69023

   Purpose

   Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   V206K monomerized super folder GFP

   Expression

   Mammalian

   Mutation

   delete codons 258-381 and fuse in frame to monome…

   Promoter

   CMV

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGIPz-PD-L1 4NQ

   Plasmid

   #121487

   Purpose

   Expression of human PD-L1 4NQ with PD-L1 shRNA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Inserts

   PD-L1 4NQ
   hPD-L1 shRNA
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N35Q, N192Q, N200Q and N219Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-FLAG-GPD1L

   Plasmid

   #32486

   Depositor

   Pere Puigserver

   Article

   Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

   Insert

   GPD1L (Gpd1l Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. His-ATG3

   Plasmid

   #202777

   Purpose

   ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabepuro3-p18Flag

   Plasmid

   #24933

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   P18 (CDKN2C Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

6. Frt-V5-hspB6

   Plasmid

   #63107

   Purpose

   Expression of HSPB6 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb6 (HSPB6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF148 pSOD1G37RAcGFP1

   Plasmid

   #26409

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G37R

   Promoter

   Available since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

8. proE-cad670-Luc-mEbox

   Plasmid

   #42084

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   E-cadherin (CDH1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   contains E-cadherin promoter region from -670 to …

   Promoter

   E-cadherin

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Frt-V5-hspB4

   Plasmid

   #63105

   Purpose

   Expression of HSPB4 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb4 (CRYAA Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tet-pLKO shMKP1.v1 puro

    Plasmid

    #87790

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid

    #136452

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NC3 pCDNA3.1 FLAG WTX (1-804)

    Plasmid

    #36958

    Depositor

    Randall Moon

    Article

    Major et al Science. 2007 May 18;316(5827):1043-6.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSI-check2-CUL1-3'UTR

    Plasmid

    #97156

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    CUL1 3'UTR (CUL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgNT.2#2/Cre

    Plasmid

    #173661

    Purpose

    Expresses a NT.2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-EZH2-T487A mutant

    Plasmid

    #28061

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T487A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCPmacroH2A.1-GS3

    Plasmid

    #204759

    Purpose

    Expression of Cas9 and human macroH2A.1

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    macroH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF153 pcDNA3.1(+)SOD1G37R

    Plasmid

    #26399

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCDH-EF1-FHC-POLQ-S1977P

    Plasmid

    #64876

    Purpose

    lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    S1977P, mimicking the chaos1 mutation

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE-Tight-FLAG-CYLD-C601A

    Plasmid

    #60029

    Purpose

    CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    TRE

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

23. GFP-CYLD-C601A

    Plasmid

    #60028

    Purpose

    N-terminal GFP tagged catalytic inactive (C601A) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

25. SPOP-V5 (BTB+BACK)

    Plasmid

    #169723

    Purpose

    Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K, Y353E

    Promoter

    CMV

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

    Plasmid

    #191484

    Purpose

    Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBabepuro3-p18

    Plasmid

    #24935

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    changed Glu 614 to Gln

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-RO1.7-Lrat

    Plasmid

    #206345

    Purpose

    AAV plasmid expressing LRAT in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

    Insert

    Lrat (Lrat Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.7kb human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-Hygro-sgSIK3

    Plasmid

    #138699

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLX302 PCSK5-V5 puro

    Plasmid

    #98389

    Purpose

    Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBMN HA-ATG13

    Plasmid

    #186223

    Purpose

    For expression of ATG13 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX nsp1 K164A/H165A CoV2

    Plasmid

    #175514

    Purpose

    For protein expression of K164A/H165A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    K164A/H165A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX nsp1 R124A/K125A CoV2

    Plasmid

    #175513

    Purpose

    For protein expression of R124A/K125A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R124A/K125A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX C_K nsp1 K164A/H165A CoV2

    Plasmid

    #175517

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. tet-pLKO shMKP1.v2 puro

    Plasmid

    #87791

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEasyG3_hph

    Plasmid

    #184919

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDN-G1Gh

    Plasmid

    #44507

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. RK5F-YAP8SA

    Plasmid

    #125008

    Purpose

    express in mammalian cell

    Depositor

    Ruey-Hwa Chen

    Article

    Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

    Insert

    YAP8SA (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PGEX-6P1-hNF2-AR mutant

    Plasmid

    #107149

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A585W-R588K

    Promoter

    Ptac

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Frt-hspB9

    Plasmid

    #63100

    Purpose

    Expression of HSPB9 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb9 (HSPB9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-Puro-SIK2

    Plasmid

    #138696

    Purpose

    Expresses a human SIK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 human (SIK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLenti-Puro-SIK3

    Plasmid

    #138697

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSECC-sgLKB1-A

    Plasmid

    #138663

    Purpose

    Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgLKB1 mouse (Stk11 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pFLAG-CMV2 MAML LHHLL

    Plasmid

    #87681

    Purpose

    Mammalian expression vector for Maml1 with LHHLL mutation

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MAML1 (MAML1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutate 1010,1011 from DD to HH

    Promoter

    CMV

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. MSCV-miR-34b/34c

    Plasmid

    #63933

    Purpose

    MSCV vector for overexpressing mouse miR-34b/34c

    Depositor

    Lin He

    Article

    Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

    Insert

    microRNA 34b/34c (Mir34c, Mir34b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

50. pENTR CMVie-Lifeact-iRFP670 L1-R5

    Plasmid

    #84390

    Purpose

    Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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