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pDONR223_ESR1_WT

Plasmid

#82208

Purpose

Gateway Donor vector containing ESR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ESR1 (ESR1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_18GZG

Plasmid

#117228

Purpose

shble flanked by an endogenous GAPDH promoter and terminator system

Depositor

Jackie Collier

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

shble

Use

Thraustochytrid expression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DinoIII-neo

Plasmid

#155026

Purpose

Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

amino 3'-glycosyl phosphotransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-pat

Plasmid

#155028

Purpose

Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Phosphinothricin N-acetyltransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-bsr

Plasmid

#155027

Purpose

Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Blasticidin-S deaminase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Use

Tags

Expression

Mutation

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1

Plasmid

#116737

Purpose

Lentiviral expression of ESR1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

Plasmid

#129180

Purpose

Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

Expression

Mutation

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-Y537S

Plasmid

#116374

Purpose

Lentiviral expression of ESR1 Y537S

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f1

Plasmid

#158755

Purpose

Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-E380Q

Plasmid

#116368

Purpose

Lentiviral expression of ESR1 E380Q

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

E380Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-Y537N

Plasmid

#116373

Purpose

Lentiviral expression of ESR1 Y537N

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537N

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-Y537C

Plasmid

#116372

Purpose

Lentiviral expression of ESR1 Y537C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537C

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-L536Q

Plasmid

#116369

Purpose

Lentiviral expression of ESR1 L536Q

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

L536Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-E247K

Plasmid

#116367

Purpose

Lentiviral expression of ESR1 E247K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

E247K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-S463P

Plasmid

#116370

Purpose

Lentiviral expression of ESR1 S463P

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S463P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-N HPyV7

Plasmid

#37869

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPyV7 ER (HPyV7_gp4, HPyV7_gp5 Polyomavirus HPyV7)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N HPyV6

Plasmid

#37868

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPyV6 ER (HPyV6_gp4, HPyV6_gp5 Polyomavirus HPyV6)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only