We narrowed to 4,786 results for: Mos

1. NONO2_IDR-EGFP

   Plasmid

   #232758

   Purpose

   Expresses NONO2_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   NONO2_IDR-EGFP (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NUP98a_IDR-EGFP

   Plasmid

   #232759

   Purpose

   Expresses NUP98a_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   NUP98a_IDR-EGFP (NUP98 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NUP98b_IDR-EGFP

   Plasmid

   #232760

   Purpose

   Expresses NUP98b_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   NUP98b_IDR-EGFP (NUP98 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. dCas9-EWSR1_IDR-EGFP

   Plasmid

   #232762

   Purpose

   Expresses dCas9-EWSR1_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-EWSR1_IDR-EGFP (EWSR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dCas9-SS18_IDR-EGFP

   Plasmid

   #232763

   Purpose

   Expresses dCas9-SS18_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-SS18_IDR-EGFP (SS18 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dCas9-NONO1_IDR-EGFP

   Plasmid

   #232764

   Purpose

   Expresses dCas9-NONO1_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-NONO1_IDR-EGFP (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT3TS-RfxCas13d-hei

   Plasmid

   #234485

   Purpose

   Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   myc and oNLS

   Expression

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-SET(1-218)

   Plasmid

   #234707

   Purpose

   expression of GFP in fusion with a C-terminally truncated SET protein

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   SET (SET Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   expresses only the N-terminal SET part (1-218)

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-SAM-Puro

   Plasmid

   #198713

   Purpose

   For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   EF1a-MPH-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSF_his6_sumo_ZIKV_NS5_MTase

    Plasmid

    #213529

    Purpose

    Expresses the MTase domain of NS5 of ZIKV

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    ZIKA virus NS5 methyltransferase domain
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    MTase domain aa 1-265

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. macroH2A2-GFP (pc2191)

    Plasmid

    #223709

    Purpose

    Expression of macroH2A2 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. macroH2A1.2-F192V-GFP (pc5115)

    Plasmid

    #223438

    Purpose

    Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.2-F192V (Macroh2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of phenylalanine 192 to valine (F192V)

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

    Plasmid

    #217802

    Purpose

    Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

    Plasmid

    #217806

    Purpose

    Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

    Plasmid

    #217790

    Purpose

    Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

    Plasmid

    #217791

    Purpose

    Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

    Plasmid

    #217792

    Purpose

    Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

    Plasmid

    #217793

    Purpose

    Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-SwCV-nCas9-EcKlenow (JO1270)

    Plasmid

    #217794

    Purpose

    Variant CE1 construct with SwCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    SwCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HUHe variant click editor (CE1) - pCMV-T7-RaCV-nCas9-EcKlenow (JO1268)

    Plasmid

    #217795

    Purpose

    Variant CE1 construct with RaCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid

    #217799

    Purpose

    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HUHe variant click editor (CE1) - pCMV-T7-BDFV-nCas9-EcKlenow (JO1271)

    Plasmid

    #217785

    Purpose

    Variant CE1 construct with BDFV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    BDFV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

    Plasmid

    #217786

    Purpose

    Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

    Plasmid

    #217787

    Purpose

    Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid

    #217788

    Purpose

    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid

    #217789

    Purpose

    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcdna 3.1 DSG-3 Tailless Control

    Plasmid

    #182718

    Purpose

    expresses a truncated force-insensitive DSG-3 TSmod

    Depositor

    Daniel Conway

    Article

    Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

    Insert

    desmoglein-3 (Dsg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TSmod inserted at G726, downstream C-terminal tai…

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. phH1-GBX2-gRNA

    Plasmid

    #192510

    Purpose

    Contains gRNA for GBX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    GBX2 (GBX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phU6-CDX4-gRNA

    Plasmid

    #192511

    Purpose

    Contains gRNA for CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX4 (CDX4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmU6-CDX1-gRNA

    Plasmid

    #192512

    Purpose

    Contains gRNA for CDX1

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX1 (CDX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ph7SK-CDX2-gRNA

    Plasmid

    #192513

    Purpose

    Contains gRNA for CDX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX2 (CDX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV2ss-U6-sgHTT1-7sk-sgCas9

    Plasmid

    #190900

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (HTT Human, spCas9)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAVss-U6-sgHTT51-7sk-Cas9

    Plasmid

    #190901

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (Htt Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

    Plasmid

    #190902

    Purpose

    AAV vector expressing thew GFP reporter gene and human sgHTT

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    GFP and sgHTT (HTT Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBK-CMV-mpx

    Plasmid

    #191826

    Purpose

    mpx probe generation for in situ

    Depositor

    Jason Berman

    Article

    Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

    Insert

    mpx cDNA (mpx Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCPF101-pfQC-cyclase dead

    Plasmid

    #184878

    Purpose

    expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

    Depositor

    Ferenc Scheeren

    Article

    Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    pfQC-cyclase dead
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    F77A & Q79A

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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38. SOD1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176964

    Purpose

    Mammalian expression vector encoding SOD1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX2T-TEV CHMP1B 186-199

    Plasmid

    #108285

    Purpose

    Expresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 186-199

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX2T-TEV CHMP1B 144-178

    Plasmid

    #108287

    Purpose

    Expresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 144-178

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid

    #108289

    Purpose

    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 188 to A, changed L 192 to A

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid

    #108291

    Purpose

    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 192 to A, changed L 195 to A

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. BPK1520 sgCHMP1A

    Plasmid

    #169321

    Purpose

    Expresses CHMP1a targeting sgRNA in non-lentiviral BPK1520 plasmid

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    sgCHMP1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BPK1520 sgCHMP4B

    Plasmid

    #169322

    Purpose

    Expresses CHMP4B targeting sgRNA in non-lentiviral BPK1520 plasmid

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    sgCHMP4B
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Desmoplakin I no force control (F40-based)

    Plasmid

    #118726

    Purpose

    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1952

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

    Plasmid

    #99743

    Purpose

    Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Changed both L165 and E166 to Alanine

    Promoter

    CMV

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pFB2

    Plasmid

    #195282

    Purpose

    Bicistronic DTR and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Puro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge