We narrowed to 30,488 results for: ica

1. GFP-α-cateninA-

   Plasmid

   #229698

   Purpose

   Transient expression of GFP-alpha-cateninA- in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L785A, I792A, V796A

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CS-TRE-BMI1-Ubc-rtTA

   Plasmid

   #198191

   Purpose

   Generation of Expandable megakaryocyte cell lines from human iPS cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

   Insert

   hBMI1 (BMI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CS-TRE-c-Myc-Ubc-rtTA

   Plasmid

   #198190

   Purpose

   Generation of Expandable megakaryocyte cell lines from human iPS cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

   Insert

   hC-MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-JEDI-2P-WPRE

   Plasmid

   #179460

   Purpose

   Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV1

   Insert

   JEDI-2P
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac Dual wt p110α/p85α

   Plasmid

   #199302

   Purpose

   co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

   Depositor

   Peter Vogt

   Article

   Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

   Insert

   His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
   

   Use

   Tags

   Avi and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-GCaMP5G

   Plasmid

   #31788

   Purpose

   a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

   Depositor

   Douglas Kim, Loren Looger, GENIE Project

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   GCaMP5G
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-α-cateninΔβH

   Plasmid

   #229705

   Purpose

   Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-α-cateninA+

   Plasmid

   #229697

   Purpose

   Transient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin binding

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 670-673, RAIM--> GSGS

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-α-cateninA+ΔβH

    Plasmid

    #229704

    Purpose

    Transient expression of GFP-alpha-cateninA+deltabetaH in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-α-cateninWT

    Plasmid

    #229709

    Purpose

    Transient expression of GFP-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-JEDI-2P-WPRE

    Plasmid

    #179464

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CS-TRE-BclXL-Ubc-rtTA

    Plasmid

    #198189

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBCLXL (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. COVID-SARS2 NSP14/10

    Plasmid

    #159613

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

    Depositor

    Opher Gileadi

    Article

    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

    Inserts

    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6, TEV cleavage site and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMEM230-WT-DsRed(isoform 2)

    Plasmid

    #99563

    Purpose

    mammalian expression of TMEM230-dsRed (isoform 2)

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid

    #112677

    Purpose

    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Nuclear-localized floxed-mCherry EGFP
    

    Use

    AAV

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

    Plasmid

    #179465

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-JEDI-2P-Kv-WPRE

    Plasmid

    #179463

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLViN-iRFP670-α-cateninWT

    Plasmid

    #229702

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid

    #105127

    Purpose

    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

    Depositor

    Kimberley Evason, Didier Stainier

    Article

    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

    Inserts

    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    cryaa (crystallin) and fabp10a

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-syn-AICD-IRES-hrGFP

    Plasmid

    #107548

    Purpose

    high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    human synapsin

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLViN-iRFP670-α-cateninA+

    Plasmid

    #229703

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid

    #217766

    Purpose

    Fluorescent reporter for ceramide (for bacterial expression and purification)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    T7 promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pMXs-GNL

    Plasmid

    #188232

    Purpose

    Polycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Insert

    Glis1, NANOG and LIN28A separated by 2A sequence (GLIS1, NANOG, LIN28A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-GFAP-JEDI-2P-WPRE

    Plasmid

    #179469

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1/Puro-CAG-JEDI-2P-Kv

    Plasmid

    #179462

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P-Kv
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Mecp2

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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