We narrowed to 9,389 results for: Coli

1. pSrpR-S1

   Plasmid

   #74691

   Purpose

   SrpR-S1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSS1-SrpR-PSrpR-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLmrA-N1

   Plasmid

   #74684

   Purpose

   LmrA-N1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSN1-LmrA-PLmrA-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIcaRA-I1

   Plasmid

   #74682

   Purpose

   IcaRA-I1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSI1-IcaRA-PIcaR-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCURE

   Plasmid

   #23013

   Depositor

   James Bowie

   Article

   Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

   Insert

   I-CreI homing endonuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I-CreI expression is controlled by the arabinose …

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. GFP Q157TAG

   Plasmid

   #68297

   Purpose

   Q157TAG GFP reporter

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   GFP Q157TAG
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKL12-GCaMP6f-pHuji bb118

   Plasmid

   #158981

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-pHuji
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833 a…

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXN-FBLWLF-LexA

   Plasmid

   #26266

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   LexA
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAL294

   Plasmid

   #167834

   Purpose

   Encodes OptoLAC1 circuit

   Depositor

   Jose Avalos

   Article

   Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

   Insert

   lacI
   

   Use

   Synthetic Biology

   Tags

   ssrA (LAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMAL296

   Plasmid

   #167835

   Purpose

   Encodes OptoLAC2 circuit

   Depositor

   Jose Avalos

   Article

   Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

   Insert

   lacI
   

   Use

   Synthetic Biology

   Tags

   ssrA (AAV)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSrpR-S2

    Plasmid

    #74692

    Purpose

    SrpR-S2 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSS2-SrpR-PSrpR-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJM.718

    Plasmid

    #108519

    Purpose

    PBetI-YFP Reporter. Cho sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PBetI-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAJM.1459

    Plasmid

    #108521

    Purpose

    P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    P3B5C-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAH-CTX1-rha

    Plasmid

    #129390

    Purpose

    Derived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Rhamnose-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBM3R1-B2

    Plasmid

    #74679

    Purpose

    BM3R1-B2 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSB2-BM3R1-PBM3R1-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBM3R1-B1

    Plasmid

    #74678

    Purpose

    BM3R1-B1 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSB1-BM3R1-PBM3R1-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSrpR-S4

    Plasmid

    #74694

    Purpose

    SrpR-S4 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSS4-SrpR-PSrpR-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBM3R1-B3

    Plasmid

    #74680

    Purpose

    BM3R1-B3 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSB3-BM3R1-PBM3R1-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJM.719

    Plasmid

    #108520

    Purpose

    PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTtg-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAJM.711

    Plasmid

    #108512

    Purpose

    PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PPhlF-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMAL658

    Plasmid

    #167837

    Purpose

    Encodes OptoLAC1B circuit

    Depositor

    Jose Avalos

    Article

    Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

    Insert

    lacI
    

    Use

    Synthetic Biology

    Tags

    ssrA (LAA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMAL659

    Plasmid

    #167838

    Purpose

    Encodes OptoLAC2B circuit

    Depositor

    Jose Avalos

    Article

    Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

    Insert

    lacI
    

    Use

    Synthetic Biology

    Tags

    ssrA (AAV)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKS1

    Plasmid

    #24636

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    'tesA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pML3009

    Plasmid

    #193256

    Purpose

    Gamma-aminobutyric acid sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    gabR
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    synthetic promoter P2 and synthetic promoter PGab…

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJB028

    Plasmid

    #65469

    Purpose

    pTrc plasmid expressing p14*-TEV

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    p14*-TEV (TEVgp1 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDF-EcAvs2

    Plasmid

    #188804

    Purpose

    Codon optimized EcAvs2 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKS011

    Plasmid

    #65464

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    MBP-Ntag-mCherry
    

    Use

    Tags

    FLAG and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGR-ECK120033127

    Plasmid

    #46011

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120033127 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKB239

    Plasmid

    #170034

    Purpose

    Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Granulysin (GNLY Synthetic, Human, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSB1A3-AD009

    Plasmid

    #116850

    Purpose

    Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

    Depositor

    Friedrich Simmel

    Article

    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

    Inserts

    AraC
    alpha-HL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDR-GW FLIPglu-170nΔ13V

    Plasmid

    #27999

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-170nΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pBP-TL5

    Plasmid

    #72989

    Purpose

    RBS_M2

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    M2 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pBP-TL11

    Plasmid

    #72995

    Purpose

    RBS_L4

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L4 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pDOC-K-glmS-lacIZ

    Plasmid

    #158061

    Purpose

    Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    lacIZ operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSET FLIPglu-170nDelta13

    Plasmid

    #13562

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCYB-TnsA

    Plasmid

    #17356

    Depositor

    Nancy Craig

    Article

    Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

    Insert

    TnsA (tnsA E. coli transposon Tn7)
    

    Use

    Tags

    Intein-Chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJAT13

    Plasmid

    #18986

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    promoter CP13
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pSEL2

    Plasmid

    #23012

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    Kanamycin resistance
    

    Use

    Tags

    6 His Tag and Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge