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Showing: 1481 - 1500 of 114172 results

  4. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

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  7. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Article
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119164-plasmid-map-sequence-id-232464

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  10. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

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  12. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138146-plasmid-map-sequence-id-272533

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  15. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid
    #80768
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
    Depositor
    Kazuhisa Nakayama
    Article
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    80768-plasmid-map-sequence-id-171038

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Showing: 1481 - 1500 of 114172 results