We narrowed to 18,137 results for: Met;

1. pCIneo-lambdaN-HA-HsDCP1a_G

   Plasmid

   #146400

   Purpose

   Mammalian Expression of HsDcp1a

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDcp1a (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and G1716T compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #106895

   Purpose

   Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF711-pET28a-Hs-delta25-MCAD-NHis wt

   Plasmid

   #85112

   Purpose

   expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta25) MCAD (ACADM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-PGK-SPdCas9-BFP-EGFP

   Plasmid

   #66821

   Purpose

   dCas9 fused to BFP and EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-EGFP
   

   Use

   Tags

   dSpCas9-BFP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pdest dTomato Puro

   Plasmid

   #223054

   Purpose

   dTomato gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   dTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. rag2:mcherry_CE_pISceI

   Plasmid

   #223047

   Purpose

   Express m-Cherry gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   m-Cherry
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. rag2:CTDSP2_CE_pISceI

   Plasmid

   #223033

   Purpose

   Express CTDSP2 gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   CTDSP2
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRoC30_Th3.1

   Plasmid

   #188065

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJRoC30_Th3.7

    Plasmid

    #188066

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJRoC30_Th3.10

    Plasmid

    #188067

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCrhaB2_ApyOAHIDS

    Plasmid

    #226246

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

    Plasmid

    #213535

    Purpose

    Expresses the split transcription factors in cultured neuron and mouse brain

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
    

    Use

    AAV

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKG-GW2-NgBR

    Plasmid

    #203165

    Purpose

    Yeast expression vector for hNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426-Cup1p-FusionRed

    Plasmid

    #188392

    Purpose

    Cu2+-dependent expression of red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

    Plasmid

    #146407

    Purpose

    Bacterial Expression of HsDCP1a_539-582

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G

    Plasmid

    #146409

    Purpose

    Bacterial Expression of HsDCP1a_539-582-mut2

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582-mut2 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G

    Plasmid

    #146411

    Purpose

    Bacterial Expression of HsDCP1a_539-582

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GA46

    Plasmid

    #196614

    Purpose

    Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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