We narrowed to 17,688 results for: SHE

1. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available Since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

2. PB-3-FT-ENGRAM

   Plasmid

   #179158

   Purpose

   piggyBac plasmid for 3'-FT ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Insert

   Csy4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   minP

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. trPAGmEos3.2

   Plasmid

   #203752

   Purpose

   Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trPAG (PAG1 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   TRC

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. piGECI-N1

   Plasmid

   #160421

   Purpose

   Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HBT-pcoCas9

   Plasmid

   #52254

   Purpose

   transient expression of pcoCas9 gene in plant cells

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   pcoCas9
   

   Use

   CRISPR; Plant expression

   Tags

   FLAG and NLS

   Promoter

   hybrid constitutive promoter 35SPPDK

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Promoter

    opposing T7 promoters

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBITE-EGFRvIII-F265-OKT3

    Plasmid

    #190679

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST1374-N-NLS-flag-linker-Cas9-H840A

    Plasmid

    #51129

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1-sfGFP-DQNAT

    Plasmid

    #198210

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_DQNAT
    

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Expression

    Bacterial and Mammalian

    Promoter

    LTR

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TfR-pHoran4

    Plasmid

    #61547

    Purpose

    Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHoran4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-24p3/LCN2-Luc

    Plasmid

    #25463

    Depositor

    Sarah Gaffen

    Article

    Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

    Insert

    24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)
    

    Use

    Luciferase

    Mutation

    none

    Available Since

    June 25, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRc/CMV2 Dvl-2-Myc

    Plasmid

    #42194

    Depositor

    Robert Lefkowitz, Shin-ichi Yanagawa

    Article

    Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

    Insert

    Dvl 2 (Dvl2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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