We narrowed to 13,583 results for: TIM

1. pCHERRY8

   Plasmid

   #24663

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. RSV CREB M1

   Plasmid

   #22395

   Depositor

   Marc Montminy

   Article

   Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

   Insert

   CREB (Creb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Serine 133 to Alanine

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pFH67

   Plasmid

   #131766

   Purpose

   Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-PSFΔPQ

   Plasmid

   #35187

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   PSF (SFPQ Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of residues 34-113

   Promoter

   CMV

   Available since

   Oct. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pEB1-Clover

   Plasmid

   #103991

   Purpose

   Plasmid encoding Clover

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Clover
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-yomKO2-CaURA3

   Plasmid

   #44881

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET FLIPglu-170nDelta13

   Plasmid

   #13562

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. KA2058_pX330A-G2P

    Plasmid

    #124202

    Purpose

    Expression of SpCas9 and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIS.MTRIA-CCK

    Plasmid

    #184602

    Purpose

    For expression of a fluorescent sensor for CCK in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-CCK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB1-Venus SX

    Plasmid

    #103990

    Purpose

    Plasmid encoding Venus SX

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Venus SX
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB1-mVenus JBC

    Plasmid

    #103987

    Purpose

    Plasmid encoding mVenus JBC

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus JBC
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Crimson/P2A-ICP8 (pSLIK1)

    Plasmid

    #113856

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-link-yoClover-SpHis5

    Plasmid

    #44857

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoClover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KA1390_pGL4-UBCmin-luc2

    Plasmid

    #124185

    Purpose

    Luciferase reporter with the UBC minimal promoter

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET FLIPglu-3.2mDelta13

    Plasmid

    #13560

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN_35S/mApple

    Plasmid

    #132566

    Purpose

    mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mApple
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBD21_033

    Plasmid

    #109150

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBD21_036

    Plasmid

    #109153

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    mKO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBD21_034

    Plasmid

    #109151

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    TagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-link-yomWasabi-SpHis5

    Plasmid

    #44850

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomWasabi
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSET FLII275Pglu-4.6m

    Plasmid

    #13565

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A G275S-ECFP-K276R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEBB-XIAP1-351

    Plasmid

    #11560

    Depositor

    Jon Ashwell

    Article

    Yang et al Science 2000 May 5;288(5467):874-7.

    Insert

    XIAP1-351 (XIAP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 352-496

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

27. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pYES2.1-cerMET17

    Plasmid

    #107443

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET17 (MET17 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-yoPSmOrange-SpHis5

    Plasmid

    #44856

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUAS-MmTFP13xmyc

    Plasmid

    #86371

    Purpose

    membrane targeted Tfp1 expression in zebrafish

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    mTfp1
    

    Use

    Zebrafish

    Tags

    3xMyc and GAP43 membrane targeting

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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