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Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v1
Plasmid#195138Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry
Plasmid#202818PurposeLentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralTagsExpressionMammalianMutationaddition of a dual aptamer RNA tag 5' to the…PromoterU6Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Integration_gRNA
Plasmid#195126PurposegRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integrationDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP11-LRRK2_G2019S
Plasmid#231175PurposeExpresses GFP-11-LRRK2 G2019S in mammalian cellsDepositorInsertGFP11-LRRK2 (G2019S) (LRRK2 Human)
UseTagsGFP11ExpressionMammalianMutationG2019SPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry
Plasmid#202822PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expressionDepositorInserttwo sgRNAs that delete exon 1 within the ZNF91 gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR2M4_Deletion_gRNA
Plasmid#195194Purposedual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFPDepositorInsertOR2M4 dual gRNA (OR2M4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO-CDK5RAP2
Plasmid#202840PurposeDoxycycline inducible expression of CDK5RAP2 cDNADepositorInsertCDK5RAP2 (CDK5RAP2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (BB)-2A-GFP-RB1sg
Plasmid#229857Purposesingle guide RNA targeting human RB1 geneDepositorInsertRB1sgRNA (RB1 Human)
UseTagsThe gamma-tubulin sgRNA is coexpressed with 3xFLA…ExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP11-LRRK2
Plasmid#231174PurposeExpresses GFP-11-LRRK2 (wildtype, full length) in mammalian cellsDepositorInsertGFP11-LRRK2 (LRRK2 Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-CDK5RAP2-SVA
Plasmid#202828PurposepGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertintronic SVA within CDK5RAP2 gene
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-AGPAT3-SVA
Plasmid#202827PurposepGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertintronic SVA located in AGPAT3 gene
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-ZNF91-BFP
Plasmid#202823PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
Plasmid#202800PurposeLentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralTagsExpressionMammalianMutationPromoterEFalpha (with intron)Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Proximal_gRNA
Plasmid#195127PurposegRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controlsDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-C
Plasmid#156471PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-C (ORF1ab Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.27
Plasmid#99362PurposeBacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationGlutamic acid 129 to LysinePromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only