We narrowed to 21,668 results for: Tes
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Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG
Plasmid#217711PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA1 (ADGRA1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-6PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-16
Plasmid#160716PurposeT-nos Terminator bordered by BsaI restriction sitesDepositorInsertnopaline synthase terminator (T-nos) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
Plasmid#223287PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.DepositorInsertmyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
UseSynthetic Biology; Piggybac transposase insertabl…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_ABE_D3_empty
Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
UseTags9xMycExpressionYeastMutationPromoterpADH1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK
Plasmid#29689PurposeGFP reporter protein fused to three GSK3 phosphorylation sitesDepositorInsertGFP glycogen synthase kinase 3 recognition site primed by MAPK
UseTagsFlag and Flag-EGFPExpressionMammalianMutationrecognition sites only, and E3 ligase sitePromoterSP6Available sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorInsertSox10 (Sox10 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_MYRF
Plasmid#64843PurposeA lentiviral vector for the expression of mouse MYRF under doxycyline control.DepositorInsertMYRF (Myrf Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT-mGold
Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP-Bulged-miR-19
Plasmid#91976PurposeEGFP reporter cell line with eight bulged miR-19 sitesDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc2 mAbp1
Plasmid#26719DepositorInsertActin-binding Protein-1 (Dbnl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-0.349cldn15la
Plasmid#125026PurposeFive prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.DepositorInsertClaudin 15-like a (cldn15la Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRT_TO_TurboID-mCherry-Cry2
Plasmid#166504PurposeExpression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.DepositorInsertNone
UseTagsTurboID and mCherry-CRY2ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-Tight-Puro_Nkx2.2
Plasmid#64840PurposeA lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.DepositorInsertNkx2.2 (Nkx2-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KR
Plasmid#66166PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by arginine residues.DepositorInsertFoxO1 KR (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242R, K245R and K262RPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KA
Plasmid#66164PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by alanine residues.DepositorInsertFoxO1 KA (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242A, K245A and K262APromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KQ
Plasmid#66165PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.DepositorInsertFoxO1 KQ (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242Q, K245Q and K262QPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB1 CTF-FLAG
Plasmid#217681PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB1 (ADGRB1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-926PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/EV
Plasmid#120833PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-HA-GFP11
Plasmid#177719PurposeER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_eCB2.0
Plasmid#164609PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in astrocytesDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterGfaABC1DAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only