162,999 results

1. pLenti CMV TRE3G Neo GFP-Progerin

   Plasmid

   #118710

   Purpose

   Lentiviral TET-ON inducible GFP-Progerin

   Depositor

   Tom Misteli

   Article

   Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

   Insert

   GFP-Progerin (LMNA Human)
   

   Use

   Lentiviral; Tet-on inducible

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G (TET-ON)

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

   Plasmid

   #166055

   Purpose

   Expresses the full length TurboID on the surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUW-tetO-Irf8

   Plasmid

   #139838

   Purpose

   doxycycline-inducible expression of mouse Irf8 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Irf8 (Irf8 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. P521_VSX2_p2A-h2b_mRuby3

   Plasmid

   #239104

   Purpose

   Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

   Depositor

   Karl Wahlin

   Article

   Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

   Insert

   VSX2 (VSX2 Human)
   

   Use

   CRISPR

   Tags

   -p2A-h2b-mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous VSX2 promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. P530_SIX6-p2A-h2b-EGFP

   Plasmid

   #239101

   Purpose

   Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

   Depositor

   Karl Wahlin

   Article

   Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

   Insert

   SIX6 (SIX6 Human)
   

   Use

   Donor plasmid

   Tags

   p2A-h2b-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous SIX6 promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV GFP DEST (736-1)

   Plasmid

   #19732

   Purpose

   3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNF02-mLychee

    Plasmid

    #219400

    Purpose

    Constitutive expression of mLychee in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLychee
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNAPf-ADRβ2 Control Plasmid

    Plasmid

    #101123

    Purpose

    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3-CD14

    Plasmid

    #13645

    Depositor

    Doug Golenbock

    Insert

    CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TLR7-YFP

    Plasmid

    #13022

    Depositor

    Doug Golenbock

    Insert

    TLR7 (TLR7 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-CMV-AfmW3A

    Plasmid

    #133402

    Purpose

    Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Afamin and Wnt3a (Afm, Wnt3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFR L858R

    Plasmid

    #11012

    Purpose

    Retroviral construct for expressing human EGFR with L858R mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR L858R (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R (tumor-derived)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSET-mSA

    Plasmid

    #39860

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_CMV:Flag-Antares

    Plasmid

    #208852

    Purpose

    Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    Antares
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNF02-mLemon

    Plasmid

    #219399

    Purpose

    Constitutive expression of mLemonin E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLemon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-SRm160

    Plasmid

    #11305

    Depositor

    Phil Sharp

    Article

    Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

    Insert

    SRm160 (SRRM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tet-off TauRD (P301L/V337M)

    Plasmid

    #188572

    Purpose

    Dox-repressible expression of the Tau repeat domain

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    Tau repeat domain (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

    Plasmid

    #128268

    Purpose

    Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Inserts

    MitoTrap
    FKBP(III)-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3 Flag p38 alpha

    Plasmid

    #20351

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CT752

    Plasmid

    #233658

    Purpose

    Flp GFP Reporter; FRT-STOP-FRT-GFP

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    FRT-STOP-FRT-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CT769

    Plasmid

    #233661

    Purpose

    Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    PhyA-Flp-397-Flp-396-FHY1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXJ-Nanoluc (Nluc)

    Plasmid

    #232656

    Purpose

    Nanoluc luciferase expression driven CMV promoter

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    Nanoluc
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXR002: EF1a-dCasRx-2A-EGFP

    Plasmid

    #109050

    Purpose

    Catalytically inactive dCasRx with 2A-EGFP

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLViN-E-cadherin-GFP

    Plasmid

    #229710

    Purpose

    Lentiviral expression of E-cadherin-GFPTG in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CDH1 E-CADHERIN Human (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmCherry Paxillin

    Plasmid

    #50526

    Depositor

    Kenneth Yamada

    Insert

    PXN paxillin (PXN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Use

    Tags

    Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. phGluc

    Plasmid

    #22522

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLKO-Tet-On-YAP1-shRNA1

    Plasmid

    #176846

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTRIP-hPGK-Blast-2A-STING-TurboID

    Plasmid

    #204713

    Purpose

    Expresses C-terminal TurboID tagged human STING for proximity ligation.

    Depositor

    Nir Hacohen

    Article

    Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

    Insert

    STING1 (STING1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge