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Plasmid#199364PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pNIC28-BSA4-G-L11
Plasmid#127830PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_TBX5_P2A_Hygro_Barcode
Plasmid#120486PurposeBarcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-CibN
Plasmid#60552PurposeExpresses dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cGR2176
Plasmid#188258PurposePlasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcGR2176
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdL
Plasmid#80938PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Dead
Plasmid#173073PurposeDead SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
UseTagsmCherry-SNAP-SNAP-VariedExpressionMammalianMutationAA144 in both SNAP tags changed from a cysteine t…PromoterCMV IE94Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
All-in-one split-ADAR2
Plasmid#170136PurposeAAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signalDepositorInsertADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-cr in PBCSK-
Plasmid#126641PurposeCloning vector LbCas12a DR-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-8-G
Plasmid#124675PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUp95GW (B7)
Plasmid#74016Purposelentiviral expression of shRNA resistant Psd95-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(1,20,6)-ADAR2
Plasmid#124620PurposeExpresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2DepositorInsertADAR2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC42
Plasmid#66564PurposesgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherryDepositorInsertssgRNA + 1XPP7
PCP-VP64 IRES mCherry
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP
Plasmid#199361PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP
Plasmid#199362PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rhoA WT
Plasmid#61631Purposemammalian expression of rhoADepositorInsertrhoA (RHOA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1e
Plasmid#134905PurposeExpression of rat Myo 1e (Myr 3) in mammalian cells.DepositorInsertMyo 1e (rat) with 3' UTR (Myo1e Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TPC2-D276K-G-GECO1.2
Plasmid#207142PurposeFusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationChanged Aspartate 276 to LysinePromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir421-CMV2-mir1
Plasmid#164673PurposeExpress miR-421 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-UAG site)
Plasmid#170138PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(UAG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN2AG5kwh
Plasmid#128253PurposeMammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.DepositorInsertBeta Globin Intron::hTetR::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQsupR-Mi2b
Plasmid#15379DepositorInsertshort hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld(FKBP F36V)-GFP
Plasmid#53260PurposepEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld(FKBP F36V)-GFP
UseTagsEGFP, Flag, and HAExpressionMutationFKBP F36VPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
tetO7_Ef1a_SOX2
Plasmid#18919DepositorInsertSOX2 (SOX2 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
PumC
Plasmid#87321PurposePumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the Pumby for C)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind CPromoterUBCAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PH-miRFP-FRB
Plasmid#193568PurposeExpresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and miRFP703ExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MAPKAP1.1-FHH-IRES-Puro
Plasmid#120705PurposeExpresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 Isoform 1
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q, N496F)
Plasmid#170139PurposeAAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signalDepositorInsertNES-MCP-ADAR2-DD(E488Q, N496F)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPA-RL-FL
Plasmid#78863PurposeDULIP Tandem Construct used for data normalizationDepositorInsertFirefly
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only