Addgene

We narrowed to 17,141 results for: puro

Showing: 3081 - 3120 of 17141 results

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pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/E37A-IRES-Puro

Plasmid

#120572

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

Plasmid

#117325

Purpose

CRISPR-Cas9 for miR-124-2/3, 3'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-2/3-3' (MIR124-3 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

Plasmid

#117324

Purpose

CRISPR-Cas9 for miR-124-1, 3'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1-3' (MIR124-1 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

Plasmid

#117323

Purpose

CRISPR-Cas9 for miR-124-3, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-3-5' (MIR124-3 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

Plasmid

#117322

Purpose

CRISPR-Cas9 for miR-124-1/2, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1/2-5' (MIR124-1 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-E383K

Plasmid

#113731

Purpose

Express mutant RGS7- E383K- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E383K

Promoter

CMV

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-MCS2-EF1-Puro-RGS7-R44C

Plasmid

#113729

Purpose

Express mutant RGS7- R44C- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-GFP-PGK-Puro

Plasmid

#110863

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-del(TAD)

Plasmid

#105597

Purpose

retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB without TAD domain (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

deletion of TAD (AA 275-327)

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E-S831E

Plasmid

#92002

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode49

Plasmid

#83726

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode49

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode42

Plasmid

#83719

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode42

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode18

Plasmid

#83695

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode18

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode92

Plasmid

#83769

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode92

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode90

Plasmid

#83767

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode90

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode28

Plasmid

#83705

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode28

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode15

Plasmid

#83692

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode15

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Tags

Expression

Mutation

please view depositor comments below

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode7

Plasmid

#83684

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode7

Use

Retroviral

Tags

Expression

Mutation

L119P in GFP

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode81

Plasmid

#83758

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode81

Use

Retroviral

Tags

Expression

Mutation

deletion of T in starting ATG of GFP (please see …

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode71

Plasmid

#83748

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode71

Use

Retroviral

Tags

Expression

Mutation

deletion of AA5-233 in GFP (please see depositor …

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode94

Plasmid

#83771

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode94

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode95

Plasmid

#83772

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode95

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode96

Plasmid

#83773

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode96

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode80

Plasmid

#83757

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode80

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode82

Plasmid

#83759

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode82

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode84

Plasmid

#83761

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode84

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode85

Plasmid

#83762

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode85

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode86

Plasmid

#83763

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode86

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode87

Plasmid

#83764

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode87

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode88

Plasmid

#83765

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode88

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode89

Plasmid

#83766

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode89

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode91

Plasmid

#83768

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode91

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode93

Plasmid

#83770

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode93

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode67

Plasmid

#83744

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode67

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode68

Plasmid

#83745

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode68

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode69

Plasmid

#83746

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode69

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3081 - 3120 of 17141 results

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