Skip to main content
Addgene
Showing: 3851 - 3900 of 32215 results

  10. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

    Enlarge

  30. pMT957

    Plasmid
    #228293
    Purpose
    Constitutive mutant PhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    ScPGK1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228293-plasmid-map-sequence-id-457079

    Enlarge

  31. pMT1035

    Plasmid
    #228300
    Purpose
    Constitutive expression of LuxTA mutant with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    luxTA mutant
    Use
    Synthetic Biology
    Tags
    Nuclear localization signal-Three copies of VP16 …
    Expression
    Yeast
    Mutation
    S116Y, W201R
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228300-plasmid-map-sequence-id-457086

    Enlarge

  32. PatoL-OG241

    Plasmid
    #72841
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72841-plasmid-map-sequence-id-133605

    Enlarge

  41. LV-Let7d-5p

    Plasmid
    #117055
    Purpose
    Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression
    Depositor
    Michele De Palma
    Article
    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d
    Insert
    Let-7d-5p - OFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFV promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117055-plasmid-map-sequence-id-223998

    Enlarge

  43. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

    Enlarge

  46. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Tags
    Expression
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

    Enlarge

  48. pRARE-MBP-DEST

    Plasmid
    #84650
    Purpose
    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.
    Depositor
    Neil Olszewski
    Article
    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    84650-plasmid-map-sequence-id-162112

    Enlarge

  50. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

    Enlarge

Showing: 3851 - 3900 of 32215 results