19,619 results

1. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Expression

   Bacterial

   Promoter

   Arabinose-inducible pBAD

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP223

   Plasmid

   #13263

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   Plac-gam-bet-exo
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR26

   Plasmid

   #127372

   Purpose

   Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Promoter

   tight TRE promoter

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. anti-FLAG M2 light chain

   Plasmid

   #175358

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 light chain

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 light chain
   

   Tags

   N-terminal secretion signal peptide (MEAPAQLLFLLL…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

   Plasmid

   #170273

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   See Comments

   Promoter

   CMVd1

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIneoGFP-BMI1

   Plasmid

   #128328

   Purpose

   Mammalian expression vector of GFP-tagged human BMI1

   Depositor

   Yutaka Hata

   Article

   Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

   Insert

   BMI1 (BMI1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GSN-bio-His

   Plasmid

   #52067

   Purpose

   Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   GSN (GSN Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS

   Plasmid

   #12158

   Depositor

   Inder Verma

   Article

   Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX_DSB_Spectrum-V3

   Plasmid

   #192476

   Purpose

   Lentiviral DSB Spectrum V3 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, mCherry, incompleteGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tubp-Gal4 in pCaSpeR4

    Plasmid

    #17747

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Tags

    Tubulin 1alpha promoter and hsp70 PolyA

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Promoter

    Chicken beta actin (CAG)

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. tdTomato-Integrin-Beta1-N-18

    Plasmid

    #58105

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE:iRFP670-EFS:Cre-2A-GFP

    Plasmid

    #68477

    Purpose

    Inducible lentivirus expressing iRFP670, Cre, and GFP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    iRFP670
    Cre-2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. sfGFP-Peroxisomes-2

    Plasmid

    #54601

    Purpose

    Localization: Peroxisomes, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    Peroximal Targeting Signal 1 (PTS1) and sfGFP

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. p53-GFP

    Plasmid

    #11770

    Depositor

    Geoff Wahl

    Insert

    p53 (TP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-EPHA1

    Plasmid

    #23929

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EPHA1 (EPHA1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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