We narrowed to 8,586 results for: Ott

1. pAAV-hSyn-RiboL1-jGCaMP7c

   Plasmid

   #192619

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP7c
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT-GEM(0)

   Plasmid

   #62891

   Purpose

   Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-hSyn-jRex-GECO

   Plasmid

   #169259

   Purpose

   AAV transfer plasmid for neuronal expression of jRex-GECO.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   JRex-GECO
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

   Plasmid

   #62952

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LB901: pMVP/PB/Blast-DEST

   Plasmid

   #121873

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT-WT-HA-GFP11

   Plasmid

   #177719

   Purpose

   ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYFAC-CH2

   Plasmid

   #168978

   Purpose

   AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in asp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LD101: pMVP (L3-L2) eCFP + WPRE

   Plasmid

   #121775

   Purpose

   pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eCFP + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYFAC-CH4

   Plasmid

   #168980

   Purpose

   AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in asp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70

    Plasmid

    #62912

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Dlx5

    Plasmid

    #15538

    Depositor

    John Rubenstein

    Article

    Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

    Insert

    Dlx5 (Dlx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCyPet-Rac1(WT)

    Plasmid

    #22782

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (wt) (RAC1 Human)
    

    Use

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCFJ1209

    Plasmid

    #44491

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-CMV-TetO2_mTurquoise2

    Plasmid

    #113895

    Purpose

    Mammalian (inducible) expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Turquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. myc-hAtg13

    Plasmid

    #31965

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    Atg13 (ATG13 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

    Plasmid

    #62957

    Purpose

    Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4 in three phases
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62903

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LB701: pMVP/PB/mCherry-DEST

    Plasmid

    #121871

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70

    Plasmid

    #62915

    Purpose

    Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV_nLightR

    Plasmid

    #217657

    Purpose

    Expresses norepinephrine indicator nLightR under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CAG-RiboL1-jGCaMP8f

    Plasmid

    #192612

    Purpose

    AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFB-Flag-MyoD-E12

    Plasmid

    #25990

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

    Plasmid

    #62898

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LC301: pMVP/PB/Zeo-DEST

    Plasmid

    #121877

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62902

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. IY601: pMVP/Lenti/Blast-DEST

    Plasmid

    #121849

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-hIes6

    Plasmid

    #15356

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    hIes6 (INO80C Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-CMV-TetO2_mVenus

    Plasmid

    #113893

    Purpose

    Mammalian (inducible) expression of cytosolic mVenus

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PB-EF1-IRES-NEO mutant ITR

    Plasmid

    #67905

    Purpose

    PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBLO 64.1

    Plasmid

    #123120

    Purpose

    E. coli expression vector for active deltaproteobacteria CasX

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEThT-CAHS1

    Plasmid

    #90027

    Purpose

    To express His-tagged CAHS1 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYFAC-CH3

    Plasmid

    #168979

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. actc1b-mKate2-ING2

    Plasmid

    #109502

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-ING2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62948

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-CMV-CAG-GFP-EnvA

    Plasmid

    #102987

    Purpose

    Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-B19 Rabies Glycoprotein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUCHR-osTIR1-IRES-GFP

    Plasmid

    #110661

    Purpose

    Lentiviral vector for stable expression of osTIR1 and selection with GFP

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    osTIR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWL89A mmPing20

    Plasmid

    #145788

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T162C, T258A, T287C, T300A, T304A, T310A, G372A

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid

    #121750

    Purpose

    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    3x HA epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. Ub[9]-mRFP[1]

    Plasmid

    #20249

    Depositor

    Nico Dantuma

    Article

    Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

    Insert

    ubiquitin C (UBC Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    changed Stop codon 686 to Glycine

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3 S6K2 E388 D3E

    Plasmid

    #17731

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

    Promoter

    CMV

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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47. pKV015

    Plasmid

    #64766

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. p3E-RhoAb-DN

    Plasmid

    #109580

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative RhoAb

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAb (rhoab Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONR223 PAK5

    Plasmid

    #60532

    Purpose

    Donor vector for human Pak5

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

50. actc1b-mKate2-Bin1b

    Plasmid

    #109509

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Bin1b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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