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Plasmid#125071PurposeExpression of SO_4387 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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UAS:bloSwitch
Plasmid#108355PurposeUAS driven green to red switch reporter for B3 recombinase expressionDepositorInsertUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
UseZebrafish expressionTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p148
Plasmid#62616PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-CocE
Plasmid#114600Purpose"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"DepositorInsertCocE
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterJ23101Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A
Plasmid#140451PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
POT10-RFP
Plasmid#65326Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT8-RFP
Plasmid#65324Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT7-RFP
Plasmid#65323Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT6-RFP
Plasmid#65322Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR353
Plasmid#125078PurposeExpression of narL under PLtetO-1, output promoter PdcuS77DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p158
Plasmid#62617PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT11-RFP
Plasmid#65327Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS424-URR
Plasmid#65330Purposehigh copy number plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT5-RFP
Plasmid#65321Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS414-URR
Plasmid#65329PurposeCEN plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT3-RFP
Plasmid#65319Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT9-RFP
Plasmid#65325Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-HipO
Plasmid#114599PurposeStrong constitutive expression of metabolic enzyme, HipO, for cell-free expressionDepositorInsertHipO
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMf3tvtaulacZ
Plasmid#37264DepositorInsertFoxb1 (Foxb1 Mouse)
UseTagstaulacZExpressionMutationPromoterAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-IC2N-dCer3
Plasmid#191325PurposeChronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)DepositorInsertIC2N-dCer-FRB (Dync1i2 Mouse)
UseTagsExpressionMammalianMutationaa 1-237PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB238
Plasmid#170033PurposeEncodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235
Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD286
Plasmid#125074PurposeExpression of SO_4622 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD276
Plasmid#125069PurposeExpression of SO_0621 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR300.3
Plasmid#125079PurposeExpression of uhpA under PLtetO-1, output Promoter PuhpT99DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro-crRNA
Plasmid#194965PurposeThe plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)PromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR357.24
Plasmid#125109PurposeExpression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR348
Plasmid#124713PurposeExpression of narX under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS193* - LacI is truncated and likely deactivated.…PromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR358.4
Plasmid#125114PurposeExpression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121DepositorInsertsnarL(REC)-liaR(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.10
Plasmid#125106PurposeExpression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSR284
Plasmid#125093PurposeExpression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143DepositorInsertsompR(REC)-manR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR241
Plasmid#125091PurposeExpression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181DepositorInsertsompR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD279
Plasmid#125099PurposeExpression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4388(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only