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Addgene

We narrowed to 561 results for: HR2

Showing: 351 - 400 of 561 results
  1. PTH2R-Tango

    Plasmid
    #66490
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Insert
    PTH2R (PTH2R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid
    #52494
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E90R, D156N, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid
    #52495
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen

    Plasmid
    #174716
    Purpose
    Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin
    Depositor
    Insert
    Rec8 (REC8 Human)
    Use
    Retroviral
    Tags
    H2B-mScarlet and mNeonGreen
    Expression
    Mammalian
    Mutation
    hRec8: 297-506; hMeikin: 1-332, E151R, R154E
    Promoter
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pBOB-3xFlag-hGpr75-p.Gln234*

    Plasmid
    #236748
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Gln234 to STOP codon, CAA to TAA
    Promoter
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pBOB-3xFlag-hGpr75-p.Ala110fs

    Plasmid
    #236747
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Ala110fs, deletion of GCCAGTA
    Promoter
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pMV306DIG13+FFlucRT

    Plasmid
    #49998
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pMV306G13+FFlucRT

    Plasmid
    #49997
    Purpose
    Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase
    Depositor
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13 from M. marinum
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid
    #70762
    Purpose
    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)
    Depositor
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E83Q,E90R,E101S,D156N,T159C
    Promoter
    EF1a
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pHW449

    Plasmid
    #198805
    Purpose
    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.
    Depositor
    Insert
    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV/CaMKIIa::iLMO2

    Plasmid
    #129481
    Purpose
    AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter
    Depositor
    Insert
    iLMO2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 351 - 400 of 561 results