Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 24,971 results for: Spr

Showing: 351 - 400 of 24971 results

Results per page:

Sort results by:

LENTICRISPR-TPI1_sgRNA1

Plasmid

#201626

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-TPI1_sgRNA2

Plasmid

#201627

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNOC-CRISPR

Plasmid

#100008

Purpose

Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgSHOC2-1

Plasmid

#86128

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 1 targeting SHOC2 (SHOC2 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR-v2-FE-PuroR

Plasmid

#186746

Purpose

Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgCOQ2

Plasmid

#186025

Purpose

knock out COQ2 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Coenzyme Q2, Polyprenyltransferase (COQ2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgSHOC2-2

Plasmid

#86129

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 2 targeting SHOC2 (SHOC2 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Tbxa2r

Plasmid

#170304

Purpose

A knock-out vector for the mouse Tbxa2r

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Tbxa2r gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-HNRNPD_sgRNA1

Plasmid

#201598

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-GPI_sgRNA1

Plasmid

#201594

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

GPI (GPI Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-ALDOA_sgRNA2

Plasmid

#201591

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1-sgSHMT1-G3

Plasmid

#83903

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-HK2_sgRNA2

Plasmid

#201597

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SPRR2A-pFastBac I

Plasmid

#182460

Purpose

Expression His-tagged SPRR2A in insect cells

Depositor

Lora Hooper

Article

Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

Insert

small proline rich protein 2A (SPRR2A Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-PABPC1_sgRNA1

Plasmid

#201608

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PABPC1 (PABPC1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-CRISPR1

Plasmid

#120366

Purpose

Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR1

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-Nr4a1sgRNA1

Plasmid

#160945

Purpose

Guide RNA 1 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 1 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCRISPRv2-sgPLXNB2

Plasmid

#86152

Purpose

Lentivirus carrying Cas9/CRISPR for cut in human PLXNB2 gene exon 3

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Ptger1

Plasmid

#170306

Purpose

A knock-out vector for the mouse Ptger1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptger1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR sgSLC19A1

Plasmid

#102314

Purpose

genetic depletion of SLC19A1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgSLC19A1 (SLC19A1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Article

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Promoter

TEF1

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2puro-Ptgs2_Balbc

Plasmid

#170301

Purpose

A knock-out vector for the mouse Ptgs2 in Balb/c

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgGPT2_9

Plasmid

#163456

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2puro-Gnaq

Plasmid

#170302

Purpose

A knock-out vector for the mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Gnaq gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MiniCoopR mitfa:SPRED1

Plasmid

#118851

Purpose

Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

SPRED1 (SPRED1 Human, Zebrafish)

Use

Zebrafish expression, tol7

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgC1orf27-1

Plasmid

#86130

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 1 targeting C1orf27 (C1orf27 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgC1orf27-2

Plasmid

#86131

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 2 targeting C1orf27 (C1orf27 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgDnmt1_2

Plasmid

#138348

Purpose

Express guide RNA 2 for mouse Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
4CE1A cSpry1

Plasmid

#22095

Depositor

Gail Martin

Insert

Sprouty 1 (SPRY1 Chicken)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 351 - 400 of 24971 results

Results per page:

Sort results by: