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Showing: 351 - 400 of 568 results

  1. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid
    #174630
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174630-plasmid-map-sequence-id-346973

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  3. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid
    #174631
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174631-plasmid-map-sequence-id-346982

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  4. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid
    #174633
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174633-plasmid-map-sequence-id-346989

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  5. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid
    #174634
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174634-plasmid-map-sequence-id-346991

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  6. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid
    #193717
    Purpose
    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
    Use
    Tags
    2xmNeongreen-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193717-plasmid-map-sequence-id-383726

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  7. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid
    #193720
    Purpose
    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193720-plasmid-map-sequence-id-383731

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  11. pBa-MARK2-eGFP

    Plasmid
    #66706
    Purpose
    mammalian expression vector for MARK2 with a C-terminal eGFP
    Depositor
    Marvin Bentley, Gary Banker
    Insert
    MARK2 (MARK2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta actin
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    66706-plasmid-map-sequence-id-114008

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  14. mEos3.2-MCPB1-N-18

    Plasmid
    #57472
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPB1 (Capzb Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57472-plasmid-map-sequence-id-213129

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  15. mEos3.2-MCPB2-C-18

    Plasmid
    #57473
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPB2 (Capzb Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57473-plasmid-map-sequence-id-213120

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  16. mEos3.2-MCPB2-N-18

    Plasmid
    #57474
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPB2 (Capzb Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57474-plasmid-map-sequence-id-213118

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  22. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230058
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230058-plasmid-map-sequence-id-460119

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  23. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230059
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Use
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230059-plasmid-map-sequence-id-460118

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  28. PS-CFP2-MCPB1-N-18

    Plasmid
    #57237
    Purpose
    Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    MCPB1 (Capzb Mouse)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57237-plasmid-map-sequence-id-210618

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  29. pMADM-alpha

    Plasmid
    #36890
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, deleted…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36890-plasmid-map-sequence-id-46195

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  30. pGFP AH2 tensin

    Plasmid
    #50514
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.
    Insert
    Tensin (TNS1 Chicken)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    AA659--761, including actin homology 2 domain of …
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid
    #193719
    Purpose
    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
    Use
    Tags
    2xHA-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193719-plasmid-map-sequence-id-383790

    Enlarge

  33. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid
    #193721
    Purpose
    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193721-plasmid-map-sequence-id-383728

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  44. p140Tol2-Olactb:SABER

    Plasmid
    #226829
    Purpose
    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Article
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Inserts
    BFP-SABER
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    226829-plasmid-map-sequence-id-455504

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  45. GFP Cav2.2 W391A pRK5

    Plasmid
    #206084
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding
    Depositor
    Annette Dolphin
    Article
    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    206084-plasmid-map-sequence-id-412224

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  46. pMRX-IP-muGFP-hATG2A mLIRΔWIR

    Plasmid
    #215508
    Purpose
    Expresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.
    Depositor
    Noboru Mizushima
    Article
    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26
    Insert
    Autophagy related 2A (ATG2A Human)
    Use
    Retroviral
    Tags
    muGFP
    Expression
    Mammalian
    Mutation
    P656R (natural variant with no functional relevan…
    Promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215508-plasmid-map-sequence-id-436479

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Showing: 351 - 400 of 568 results