We narrowed to 14,168 results for: eng
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Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorPublicationAvailable sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABMutationD10A and H840AAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorPublicationInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorPublicationInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
TagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRv2 puro
Plasmid#98290PurposeVariant of lentiCRISPRv2 that confers puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF.hICN1.CMV.GFP
Plasmid#17623PurposeDepositorPublicationAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_EGFPligand
Plasmid#79129PurposeLentiviral vector for surface expressed EGFP (SFFV promoter)DepositorInsertEGFP surface ligand
UseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_Gal4UAS_tBFP_PGK_mCherry
Plasmid#79130PurposeLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherryDepositorInserttBFP
UseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCTcon2
Plasmid#41843DepositorPublicationInsertCD20
TagsHA and c-mycExpressionYeastAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-PTEN
Plasmid#13039DepositorAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)
Plasmid#139999PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorPublicationInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
Tags6XHISExpressionBacterialPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by