163,584 results

1. pRRLSIN.cPPT.PGK-GFP.WPRE

   Plasmid

   #12252

   Purpose

   3rd generation lentiviral backbone

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcVpx.myc

   Plasmid

   #213388

   Purpose

   Generate lentiviral vector which increases gene delivery into primary human myeloid cells

   Depositor

   Nathaniel Landau

   Article

   Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

   Insert

   SIVmac vpx (vpx Simian Immunodeficiency Virus)
   

   Use

   Lentiviral

   Tags

   myc-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Golgi-TurboID

   Plasmid

   #200959

   Purpose

   Transiently expresses golgi localized TurboID with HA tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Golgi-TurboID
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS_Nova

   Plasmid

   #239831

   Purpose

   NovaIscB mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 and swap o…

   Promoter

   CMV

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-ZAP70

   Plasmid

   #23887

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ZAP70 (ZAP70 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-APOBEC1-YTHmut

   Plasmid

   #178950

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTHmut-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Carol Gross, Stanley Qi, Jason Peters

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sec61b-V5-TurboID

    Plasmid

    #166971

    Purpose

    Expresses ER targeted TurboID for secretome biotinylation

    Depositor

    Hyun-Woo Rhee

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-mMLH1dn

    Plasmid

    #174825

    Purpose

    Mammalian expression of mouse MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    mouse MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 758-760

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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