159,132 results

1. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn Donor

   Plasmid

   #236211

   Purpose

   Arabinose-inducible Tn5 InducTn-seq plasmid

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

   Plasmid

   #209727

   Purpose

   Expresses cytosolic medium affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95K.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-pegRNA-puro backbone

   Plasmid

   #214085

   Purpose

   Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2 Notch1 Full Length-6MT

   Plasmid

   #41728

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Full Length (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   L1997V; Contains murine Notch-1 protein up to ami…

   Promoter

   Available since

   Dec. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-STAT3-Y705F

   Plasmid

   #74434

   Purpose

   Expression of STAT3-Y705F

   Depositor

   Jie Chen

   Article

   Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

   Insert

   Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Mutations E16K, Y705F

   Promoter

   CMV

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. phINA

    Plasmid

    #132606

    Purpose

    Expresses human alpha-internexin

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    INA (INA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Synthetic, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-tdPP7-3xmCherry

    Plasmid

    #74926

    Purpose

    Expresses tdPP7-3xmCherry

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-3xmCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xnrUAS:GFP

    Plasmid

    #61373

    Purpose

    4xnrUAS driving GFP

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnr UAS:GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid

    #141385

    Purpose

    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-E (E SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. HA-mDyn3 pcDNA3

    Plasmid

    #36265

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 3 (Dnm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TFORF0756

    Plasmid

    #141685

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge