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164,577 results

Showing: 1621 - 1640 of 164577 results

  2. pEF1-Pls4-sfGFP

    Plasmid
    #205751
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pls4 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205751-plasmid-map-sequence-id-478437

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  5. pTRE2_CMV_Mex3a_t2a_mCherry

    Plasmid
    #223244
    Purpose
    tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    amino acids conserved, underlying DNA sequence re…
    Promoter
    CMV
    Available Since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223244-plasmid-map-sequence-id-441777

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  6. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid
    #223245
    Purpose
    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    4 point mutations in RING Domain: Cys 484 Ala; Hi…
    Promoter
    CMV
    Available Since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223245-plasmid-map-sequence-id-441774

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  7. pKEK3230

    Plasmid
    #234102
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    234102-plasmid-map-sequence-id-471768

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  10. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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  13. pHW1385

    Plasmid
    #229879
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Use
    Tags
    mScarlet-I-C1
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229879-plasmid-map-sequence-id-460859

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  17. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  18. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  20. FFA2-DuET

    Plasmid
    #213238
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FFA2 (FFAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213238-plasmid-map-sequence-id-430796

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Showing: 1621 - 1640 of 164577 results