We narrowed to 8,905 results for: Ott

Showing: 3241 - 3280 of 8905 results

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p3E-CaaX(tK)

Plasmid

#109540

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Kras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BpWcbL

Plasmid

#125590

Purpose

Overexpression plasmid for production of WcbL from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al Chem Biol. 2015 Dec 17;22(12):1622-32. d

Insert

BpWcbL (wcbL Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK139A

Plasmid

#92403

Purpose

hQPRTase harbouring K139A mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV cleacable 6xHis

Expression

Bacterial

Mutation

K139A

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAOUTA4

Plasmid

#69458

Purpose

RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-OUT A4

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119 and J23119 (sigma 70)

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAINS4

Plasmid

#69456

Purpose

RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-IN S4 + SFGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

mutations for S4 variant (from wt)

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAINS3

Plasmid

#69455

Purpose

RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-IN S3

Use

Synthetic Biology

Expression

Bacterial

Mutation

mutations for S3 variant (from wt)

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAOUTA3

Plasmid

#69457

Purpose

RNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-OUT A3

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Tcrb-V13 in situ probe

Plasmid

#45622

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Tcrb-V13 in situ probe (Tcrb Mouse)

Use

In situ

Mutation

fragment contains bp# 533-1058 of X67128

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
RCAS (A) Radical Fringe (CT#475)

Plasmid

#13981

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) Radical Fringe (CT#476)

Plasmid

#13982

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) flag Radical Fringe (CT#472)

Plasmid

#13983

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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LZRS-hNEDD9_IresGFP

Plasmid

#21962

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

NEDD9 (NEDD9 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pmScarlet-i_C1

Plasmid

#85044

Purpose

Mammalian expression of mScarlet-i

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro-2A-TEVp

Plasmid

#99610

Purpose

Lentivirus expressing puromycin resistance gene and TEV protease

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Puromycin resistance (PAC gene)
TEV protease

Use

Lentiviral

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-I997A

Plasmid

#111832

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin isoleucine 997 to alanine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eNpHR-EYFP-WPRE

Plasmid

#20946

Purpose

3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

halorhodopsin

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI Probe

Plasmid

#211507

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BcPI-PLC-ANH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

MBP and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pOZITX-S1

Plasmid

#184925

Purpose

Expresses the catalytic S1 subunit of OZITX (EcPltAB)

Depositor

Jonathan Javitch

Article

Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

Insert

codon optimized S1 subunit of OZITX

Expression

Mammalian

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102G

Plasmid

#112160

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-NLuc-IRES-Puro

Plasmid

#130936

Purpose

PiggyBac vector for constitutive NanoLuc expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NanoLuc

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

Plasmid

#200277

Purpose

Characterisation of the sialic acid-responsive circuit with fluorescent reporter

Depositor

Matthew Chang

Article

Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

Inserts

CadC
NanR

Expression

Bacterial

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-CUL3

Plasmid

#19893

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 3 (CUL3 Human)

Tags

3xmyc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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DTA in pCDEST2

Plasmid

#223705

Purpose

Mammalian expression vector encoding diptheria toxin A

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

Diphtheria toxin A

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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HA Nprl3 pRK5

Plasmid

#46330

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl3 (NPRL3 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pFS462

Plasmid

#89065

Purpose

his7 integration plasmid with Z3EVpr:GFP

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter GFP-NLS leu1 C-terminal 300 nt

Use

S. pombe his7 integration vector

Promoter

Z3EV

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-Cas9-2A-EGFP

Plasmid

#167928

Purpose

Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

T2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

tTRE, hPGK

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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We narrowed to 8,905 results for: Ott