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Plasmid#72940PurposeLevel 1 Destination vector - Position BDepositorInsertLevel 1 - B site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXM03
Plasmid#121150PurposeThis plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coliDepositorInsertXimB, IDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-3.2mDelta13
Plasmid#13560DepositorInsertFLIPglu F16A S112A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A, S112A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
SepOTSκ
Plasmid#68291PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII275Pglu-4.6m
Plasmid#13565DepositorInsertFLIPglu F16A G275S-ECFP-K276R
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLBC0150
Plasmid#172795PurposeLevel 0 module containing NT2_TurboIDDepositorInsertTurboID with 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GST
Plasmid#134592PurposeLow Phosphate inducible GSTDepositorInsertGlutathione S-transferase
UseTagsnoneExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY1
Plasmid#50597PurposeBackbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,DepositorTypeEmpty backboneUseSynthetic Biology; Empty vectorTagsExpressionBacterialMutationPromoterPLacUV5Available sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPJ173(JPUB_006777)
Plasmid#83834PurposeCarries NEW operon 10 finalDepositorInsertsKuste3336
Kuste3339
Kuste3341
UseTagsExpressionMutationPromoterop10_3339_for, op10_3341_for, and op10_BsaI_3336_…Available sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ167(JPUB_006765)
Plasmid#83831PurposeCarries NEW operon 3 finalDepositorInsertsKuste2803
Kuste2804
UseTagsExpressionMutationPromoterop3_2804_for and op3_BsmBI_2803_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-2uDelta13
Plasmid#13561DepositorInsertFLIPglu F16F D152A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A, D152A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKB237
Plasmid#170030PurposeEncodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-DHFR
Plasmid#20217DepositorInsertDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-mCherry
Plasmid#127058PurposeLow phosphate inducible mCherry with N-terminal linker and His tagDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-lacZ
Plasmid#72935PurposeLevel 1 Destination vector - Position ADepositorInsertLevel 1 - A site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBEL2003
Plasmid#139888PurposeExpression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutantDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta515-746)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2000
Plasmid#139885PurposeExpression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutantDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta161-747)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishTagsExpressionMutationPromotercx55.5Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbB
Plasmid#114155PurposeExpresses full-length ClbBDepositorInsertclbB
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY4
Plasmid#53589PurposeProduces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.DepositorInsertlcyE
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMMB206-CT61
Plasmid#165410PurposeBacteria gene circuit on low copy backbone.DepositorInsertCT61 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
UseTagsExpressionBacterialMutationPromoterrhaSAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII32Pglu-2.2m
Plasmid#13566DepositorInsertFLIPglu F16A P32S-ECFP-D33R
UseTags6xHis and CitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
MazF-K56A
Plasmid#164274PurposeTo express recombinant MazF K56A point mutant proteinDepositorInsertMazF
UseTagsExpressionBacterialMutationK56APromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-yibDp-matB
Plasmid#134597PurposeLow Phosphate inducible matB (malonyl-CoA synthetase)DepositorInsertMalonyl CoA synthetase
UseTagsExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xDHFR-myc
Plasmid#20216DepositorInsert3xDHFR (folA E. coli)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
SepOTSγ
Plasmid#68284PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 1xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM911
Plasmid#72973PurposeLevel 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM911 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ppj217(JBEI-13454)
Plasmid#89787Purposefor expression of N-8xHis-StrepII-MBP-3607DepositorInsert3607
UseTags8xHis-StrepII-MBPExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNG-mCh
Plasmid#124215PurposeCytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mChDepositorInsertmNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
SepOTSε
Plasmid#68286PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 1xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPZP FLIPmal-25u
Plasmid#14476DepositorInsertFLIPmal
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationW230APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
SepOTSθ
Plasmid#68289PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 2xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23101-deGFP-T500 (131p)
Plasmid#70207PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAH0016
Plasmid#79824PurposeDual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicolDepositorInsertscat
aadA
UseTagsExpressionMutationPromoterAATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only