We narrowed to 22,137 results for: his

1. pET24a-His6-CUbo(K63R)-MCS

   Plasmid

   #212727

   Purpose

   Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET24a-His6-CUbo(K48R)-MCS

   Plasmid

   #212726

   Purpose

   Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b-His6-CUbo(K63R)-GFP

   Plasmid

   #212722

   Purpose

   Bacterial expression of His6-CUbo(K63R)-GFP

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   GFP
   

   Use

   Tags

   6xHis-CUbo(K63R)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-His6-CUbo(K48R)-GFP

   Plasmid

   #212721

   Purpose

   Bacterial expression of His6-CUbo(K48R)-GFP

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   GFP
   

   Use

   Tags

   6xHis-CUbo(K48R)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET16b-RPA4/His14 (MSW#949)

   Plasmid

   #208075

   Purpose

   Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

   Depositor

   Marc Wold

   Article

   Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

   Insert

   RPA4, RPA14(His-tagged)
   

   Use

   Tags

   10xHis tag on RPA14

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

   Plasmid

   #201552

   Purpose

   Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1
   

   Use

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   changed lysine 282 to arginine (numbering based o…

   Promoter

   Ac5

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Anti-Histone H3 pThr11 [N123/48R]

   Plasmid

   #206529

   Purpose

   Mammalian Expression Plasmid of anti-Histone H3 pThr11 (Rat). Derived from hybridoma N123/48.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Histone H3 pThr11 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hist1h2ail Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Anti-Histone H3 pThr11 [N123/19R]

   Plasmid

   #206528

   Purpose

   Mammalian Expression Plasmid of anti-Histone H3 pThr11 (Rat). Derived from hybridoma N123/19.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Histone H3 pThr11 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hist1h2ail Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE-80L 2xHis-Rab12 Q101L

   Plasmid

   #207347

   Purpose

   Bacterial expression of Rab12

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   Rab12 (RAB12 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   Q101L

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b+His SUMO Δ37 PPM1H

    Plasmid

    #207334

    Purpose

    Bacterial expression of His SUMO Δ37 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Δ37

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b+His SUMO Rab10(Q68L)

    Plasmid

    #207342

    Purpose

    Bacterial expression of His SUMO Rab10(Q68L)

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    Rab10 (RAB10 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Q68L

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-His-TEV-A7-Strep (2-180)

    Plasmid

    #190067

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV and Strep

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDL28_Cas9-His Δcys E532C/E1207C

    Plasmid

    #206289

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDL27_Cas9-His Δcys E532C/E945C

    Plasmid

    #206288

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDL26_Cas9-His Δcys M1C/E532C

    Plasmid

    #206287

    Purpose

    Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Promoter

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Promoter

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHis-3xFLAG-CMV14-CYB5R3 K214R

    Plasmid

    #204552

    Purpose

    Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    CYB5R3 (CYB5R3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K214R

    Promoter

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCI_E01_mini-mAgrin-Myc-His-WPRE_v2

    Plasmid

    #198128

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. His SUMO HNRNPA1 dHexa LCD

    Plasmid

    #197014

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

    Insert

    HNRNPA1
    

    Use

    Tags

    6His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-20b_HtrA2(delta(133))_S306A_6His

    Plasmid

    #197975

    Purpose

    E. coli expression of HtrA2(delta133)-6His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    delta(1-133), S306A

    Promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG2

    Plasmid

    #198139

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCI_E01_NtA-Fs1-Myc-His-WPRE

    Plasmid

    #198132

    Purpose

    Express NtA-Fs1 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    NtA-Fs1
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnEA-NvM-HsHelz_1475-1642-GB1His6_AH

    Plasmid

    #148890

    Purpose

    Bacterial Expression of HsHelz_1475-1642

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1475-1642 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pnEA-NvM-HsHelz_1475-1942-GB1His6_AH

    Plasmid

    #148891

    Purpose

    Bacterial Expression of HsHelz_1475-1942

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1475-1942 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pnEA-NvM-HsHelz_1643-1792-GB1His6_AH

    Plasmid

    #148893

    Purpose

    Bacterial Expression of HsHelz_1643-1792

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1643-1792 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pnEA-NvM-HsHelz_1793-1914-GB1His6_AH

    Plasmid

    #148894

    Purpose

    Bacterial Expression of HsHelz_1793-1914

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1793-1914 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pnEA-NvM-HsHELZ_1793-1942-GB1His6_AH

    Plasmid

    #148896

    Purpose

    Bacterial Expression of HsHelz_1793-1942

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1793-1942 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pnEA-NvM-HsHelz_1051-1474-GB1His6_AG

    Plasmid

    #148798

    Purpose

    Bacterial Expression of HsHelz_1051-1474

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1051-1474 (HELZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pnYC-NpM-DmRoquin_702-819-GB1His6_AE

    Plasmid

    #148624

    Purpose

    Bacterial Expression of DmRoquin_702-819

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_702-819 (roq Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pnYC-NpM-DmRoquin_501-702-GB1His6_AE

    Plasmid

    #148620

    Purpose

    Bacterial Expression of DmRoquin_501-702

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_501-702 (roq Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z

    Plasmid

    #148165

    Purpose

    Insect Expression of DmThor-CLL78AAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-CLL78AAA (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z

    Plasmid

    #148166

    Purpose

    Insect Expression of DmThor-RGTtoAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-RGTtoAAA (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z

    Plasmid

    #148167

    Purpose

    Insect Expression of DmThor-Y54AM59A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-Y54AM59A (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge