We narrowed to 6,999 results for: mme

1. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229794

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 8522-M01-663

   Plasmid

   #225660

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1a154 (H77)

   Plasmid

   #177689

   Purpose

   HCV E1E2 expression plasmid

   Depositor

   Justin Bailey

   Article

   Salas et al Gastroenterology. 2022 Feb;162(2):562-57

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SERPINC1-bio-His

   Plasmid

   #52076

   Purpose

   Expresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINC1 (SERPINC1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX458_mCherry-SRRM2-gRNA

   Plasmid

   #238251

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SRRM2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19_msfGFP-SRRM2-repair-template

   Plasmid

   #238247

   Purpose

   For knock-in msfGFP to SRRM2 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SRRM2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETSUMO2_Vitiosangium-bGSDM-thrombin

   Plasmid

   #222099

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Vitiosangium-bGSDM-thrombin ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETSUMO2_Nostoc-bGSDM-WELQ

   Plasmid

   #222100

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Nostoc-bGSDM-WELQ ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

   Plasmid

   #229788

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF10 and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229785

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229792

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-BRD4-NUT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. P2RX1-bio-His

    Plasmid

    #53359

    Purpose

    Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    P2RX1 (P2RX1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NSUN2

    Plasmid

    #188059

    Purpose

    Bacterial expression plasmid for human NSUN2; for activity testing

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-dLight1.1

    Plasmid

    #111053

    Purpose

    Expresses dLight1.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-phiC31-LP-TC

    Plasmid

    #183755

    Purpose

    AAVS1 targeting construct with the phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SERPINE2-bio-His

    Plasmid

    #53416

    Purpose

    Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINE2 (SERPINE2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NRXN-bio-His

    Plasmid

    #51810

    Purpose

    Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    NRXN (NRXN1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-2TK cyclin T1 (P#468)

    Plasmid

    #14607

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

25. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-dLight1.3b

    Plasmid

    #111056

    Purpose

    Expresses dLight1.3b in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3b
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKM-U6-reRNA-acceptor-2xBbsI

    Plasmid

    #205633

    Purpose

    Cloning and U6-drive expression of ISDra2 TnpB reRNAs

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-MEK5DD-HA

    Plasmid

    #65247

    Purpose

    Expression of HA tagged dominant active MEK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    MEK5DD (MAP2K5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Ser311 to Asp, Ser315 to Asp

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST40-MCU-V5-HIS

    Plasmid

    #31731

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Tags

    V5-HIS6X

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PDIA4-bio-His

    Plasmid

    #52071

    Purpose

    Expresses full-length Protein disulfide-isomerase A4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PDIA4 (PDIA4 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST47-MCU-GFP

    Plasmid

    #31732

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SIN40C.SFFV.dTomato.let-7c

    Plasmid

    #169301

    Purpose

    Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGIPz-PD-L1 WT

    Plasmid

    #121486

    Purpose

    2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-dLight1.2

    Plasmid

    #111054

    Purpose

    Expresses dLight1.2 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.2
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. KTK_002

    Plasmid

    #180527

    Purpose

    Entry vector containing J23104 promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    J23104 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB-cT3G-cERH-ZNF281

    Plasmid

    #222923

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZNF281 (ZNF281 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 1a138

    Plasmid

    #177688

    Purpose

    HCV E1E2 expression plasmid

    Depositor

    Justin Bailey

    Article

    Salas et al Gastroenterology. 2022 Feb;162(2):562-57

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pH3R-mCherry-N1

    Plasmid

    #84327

    Purpose

    Histamine-3 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H3R-mCherry (HRH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SERPINE1-bio-His

    Plasmid

    #52077

    Purpose

    Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINE1 (SERPINE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge