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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 14,585 results for: pET

Showing: 3281 - 3320 of 14585 results

  1. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  27. pETM-41P-HsNot1iso1_1597-2376_V

    Plasmid
    #147746
    Purpose
    Bacterial Expression of HsNot1iso1_1597-2376
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1597-2376 (CNOT1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147746-plasmid-map-sequence-id-290193

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  28. pETM-60-NdeI-NcQde-2_V

    Plasmid
    #147747
    Purpose
    Bacterial Expression of NcQde-2
    Depositor
    Elisa Izaurralde
    Insert
    NcQde-2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    1 non silent mutation P761S compared to the seque…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147747-plasmid-map-sequence-id-290194

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  33. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

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  34. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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  37. pETM-60-NcQDE2_506-938-H609A_O

    Plasmid
    #147083
    Purpose
    Bacterial Expression of NcQde-2_506-938-H609A
    Depositor
    Elisa Izaurralde
    Insert
    NcQde-2_506-938-H609A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147083-plasmid-map-sequence-id-289530

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  38. pETM-60-NcQDE2_506-938-T642E_O

    Plasmid
    #147084
    Purpose
    Bacterial Expression of NcQde-2_506-938-T642E
    Depositor
    Elisa Izaurralde
    Insert
    NcQde-2_506-938-T642E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147084-plasmid-map-sequence-id-289531

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  39. pETM-41P-DmGW182_635-861_N

    Plasmid
    #147014
    Purpose
    Bacterial Expression of DmGW182_635-861
    Depositor
    Elisa Izaurralde
    Insert
    DmGW182_635-861 (gw Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Two non silent mutations V704A and N799S and one …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147014-plasmid-map-sequence-id-289461

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  40. pETM-41P-DmGW182_700-840_N

    Plasmid
    #147015
    Purpose
    Bacterial Expression of DmGW182_700-840
    Depositor
    Elisa Izaurralde
    Insert
    DmGW182_700-840 (gw Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Two non silent mutations V704A and N799S and one …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147015-plasmid-map-sequence-id-289462

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Showing: 3281 - 3320 of 14585 results