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Showing: 3321 - 3360 of 8645 results

  2. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid
    #178090
    Purpose
    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178090-plasmid-map-sequence-id-350140

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  17. pPlacPbuCas13b-gRNA-T-MS2

    Plasmid
    #184839
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184839-plasmid-map-sequence-id-373965

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  19. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  21. LN-entry+NLS

    Plasmid
    #128011
    Purpose
    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Article
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    LN-3xFlag-SV40NLS
    Use
    Lamdan entry vector with nls to generate tetherin…
    Tags
    LN-3xFlag-SV40NLS
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128011-plasmid-map-sequence-id-251573

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  25. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

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  26. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid
    #100963
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    F138I, T78H
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100963-plasmid-map-sequence-id-193742

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  28. pDONR P4-P1R-V5-6xHis

    Plasmid
    #48346
    Purpose
    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    V5 and 6xHis epitope tags cassette
    Use
    Gateway entry clone
    Mutation
    Contains a Kozak sequence and initiation codon up…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48346-plasmid-map-sequence-id-70499

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  36. pDRF'- AmTryoshka1;3-LS-F138I

    Plasmid
    #100922
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    F138I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100922-plasmid-map-sequence-id-193743

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  37. pDRF'- AmTryoshka1;3-LS-L255I

    Plasmid
    #100964
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    L255I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100964-plasmid-map-sequence-id-193747

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  38. pDRF'- AmTryoshka1;3-GS-F138I

    Plasmid
    #100965
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    F138I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  39. pDRF'- AmTryoshka1;3-GS-L255I

    Plasmid
    #100966
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    L255I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100966-plasmid-map-sequence-id-193744

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  40. cBEST2

    Plasmid
    #234658
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    kasO*17tss and kasOp*
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234658-plasmid-map-sequence-id-492748

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Showing: 3321 - 3360 of 8645 results