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Showing: 4151 - 4200 of 9181 results

  5. pSCL.031

    Plasmid
    #184968
    Purpose
    Test effect of extending -Eco2 a1/a2 in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco2: RT and ncRNA(extended), a1/a2 length: 29
    Use
    Tags
    Expression
    Yeast
    Mutation
    human codon optimized RT, a1/a2 length extended t…
    Promoter
    Gal7
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184968-plasmid-map-sequence-id-376702

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  10. pSLS.544

    Plasmid
    #184961
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184961-plasmid-map-sequence-id-376648

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  12. pSCL.037

    Plasmid
    #184966
    Purpose
    Express -Eco1 dead RT and ncRNA in yeasst
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
    Use
    Tags
    Expression
    Yeast
    Mutation
    Human codon optimized RT, YXDD catalytic region m…
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184966-plasmid-map-sequence-id-376554

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  17. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR torR - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181946-plasmid-map-sequence-id-356600

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  18. pRB120.2

    Plasmid
    #181947
    Purpose
    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 torR - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181947-plasmid-map-sequence-id-356558

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  25. pTS2341_Tier3(SB)-Puro

    Plasmid
    #169639
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169639-plasmid-map-sequence-id-366789

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  30. pJM143

    Plasmid
    #134166
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134166-plasmid-map-sequence-id-271475

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  34. pAL800

    Plasmid
    #119306
    Purpose
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    amino acids 1 through 171 deleted, and mutatioms …
    Promoter
    lac
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119306-plasmid-map-sequence-id-232254

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  35. pAL928

    Plasmid
    #119853
    Purpose
    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysteine 169 to Serine, Cysteine 179 to Serine, C…
    Promoter
    T7
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119853-plasmid-map-sequence-id-232052

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  38. pJB03

    Plasmid
    #111961
    Purpose
    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Use
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    Tac
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111961-plasmid-map-sequence-id-219904

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  41. pTU2-D-lacZ

    Plasmid
    #72953
    Purpose
    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72953-plasmid-map-sequence-id-447522

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  42. pTU2-D-RFP

    Plasmid
    #72957
    Purpose
    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72957-plasmid-map-sequence-id-447521

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Showing: 4151 - 4200 of 9181 results