We narrowed to 11,739 results for: 002

1. I-AGCT

   Plasmid

   #43002

   Depositor

   Kiran Musunuru, Chad Cowan

   Article

   Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

   Insert

   I-AGCT
   

   Use

   Bacterial cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pMega-MaPylRS

   Plasmid

   #200225

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   proK-lacO and tacI

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOM20*-SBP-GFP

   Plasmid

   #120173

   Purpose

   Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
   

   Use

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Mutation

   Truncated TOM20: 1-30 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

   Plasmid

   #162280

   Purpose

   Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN8A Variant 1, stabilized (SCN8A Human)
   

   Use

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   Modified human HSPA5 intron 4 inserted after bp23…

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCh-KIF17*-strep

   Plasmid

   #120167

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 17 (KIF17 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF17: 1-509 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCh-KIF13A*-strep

   Plasmid

   #120166

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 13A (KIF13A Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF13A: 1-639 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-SBP-VAP-A*

   Plasmid

   #120175

   Purpose

   Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   VAMP associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP and SBP

   Expression

   Mammalian

   Mutation

   Truncated VAP-A: 118-249

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCh-KIF5A*-strep

   Plasmid

   #120163

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5A (Kif5a Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5A: 1-559 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcGFP1-N1-Dsup

    Plasmid

    #90020

    Purpose

    To express GFP-tagged Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVtrunc Affimer6-msfGFPΔN12

    Plasmid

    #231550

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PJ-DEAD

    Plasmid

    #38002

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1 (SAC1 Budding Yeast)
    INPP5E (INPP5E Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Asp 1263 mutated to Ala and Cys 779 mutated to Ser

    Promoter

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVtrunc Affimer6-sfCherry2ΔN12

    Plasmid

    #231551

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Use

    Tags

    sfCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

    Plasmid

    #203539

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-mScarlet-2A-mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-GfABC1D-SaCas9-WPRE3-pA

    Plasmid

    #203540

    Purpose

    SaCas9 expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

    Plasmid

    #203541

    Purpose

    gRNA expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-GFP, 2x U6-RNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-BG505.SOSIP-QES.i03.c01-8his

    Plasmid

    #111846

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-VTKD4-ChR2-YFP

    Plasmid

    #178721

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    ChR2-YFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAcGFP1-N1-Dsup-C

    Plasmid

    #90022

    Purpose

    To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C-terminal region alone

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge