161,600 results

1. pcDNA3 flag p53

   Plasmid

   #10838

   Depositor

   Thomas Roberts

   Article

   Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUltra

   Plasmid

   #24129

   Purpose

   3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

   Depositor

   Malcolm Moore

   Article

   Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol1-UAS:CoChR-tdTomato

   Plasmid

   #124233

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   CoChR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-VPR_P2A_mCherry

   Plasmid

   #154193

   Purpose

   Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sp-dCas9-VPR_P2A_mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1as

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN4-EF2-ABCG2-IRES-Neo

   Plasmid

   #25983

   Depositor

   Ren-he Xu

   Article

   Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

   Insert

   ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
   

   Use

   Lentiviral

   Tags

   EF1-apha promoter and IRES-Neo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. p11d-tRPA(123)

   Plasmid

   #102613

   Purpose

   Expression of human RPA in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Inserts

   RPA1 (RPA1 Human)
   RPA2 (RPA2 Human)
   RPA3 (RPA3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DJ-N589X

    Plasmid

    #130878

    Purpose

    Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    AAV-DJ cap N589X
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N589X

    Promoter

    Unknown

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mGreenLantern

    Plasmid

    #161912

    Purpose

    mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. p18sheLL

    Plasmid

    #37321

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV18L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid

    #80921

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human V5-IRF3-pcDNA3

    Plasmid

    #32713

    Depositor

    Saumen Sarkar

    Article

    Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2/shp53/GFP4

    Plasmid

    #20208

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    shp53/GFP4
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    MMLV Reverse Transcriptase
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. SPLICS Mt-ER Long P2A

    Plasmid

    #164107

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge